Objectives

This study investigated the effect of matrix metalloproteinase 8 (MMP-8) on resin–dentin bonds, assessed the mechanical properties of the interfaces over time, and discussed the potential application of MMP-8 inhibitor I (MMP8-I) as a specific MMP-8 inhibitor to be incorporated into dental adhesives.

Methods

The activation and inhibition of MMP-8 was detected by colorimetric assay. After 1 day, 6 months and 1 year of storage of Control, MMP8-I, and chlorhexidine (CHX) groups, the microtensile bond strengths (μTBS) were used to evaluate the bond strength and failure mode distributions, and nanoleakage analysis was used to evaluate the minor scattered silver particles.

Results

Colorimetric assay showed that the activated MMP-8 was enhanced by adhesive procedures, while it was inhibited by the additional treatment of MMP8-I or CHX. Compared with the Control and CHX groups, the MMP8-I group had significantly higher bond strength and the hybrid layer from the MMP8-I-treated dentin exhibited structural integrity of the collagen network and decreased silver nitrate penetration after 1 year of storage.

Significance

MMP-8 inhibition I protects against the degradation of resin–dentin bonds over time, which is better than broad-scale enzyme inhibitor CHX. It shows that MMP8-I may be used in dentistry for preventing collagen degradation within hybrid layers to extend the longevity of resin–dentin bonds.

中文翻译：

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基质金属蛋白酶8抑制剂对树脂-牙本质键的影响

目标

这项研究调查了基质金属蛋白酶8（MMP-8）对树脂-牙本质键的影响，评估了随时间变化的界面机械性能，并讨论了MMP-8抑制剂I（MMP8-I）作为特定MMP的潜在应用-8抑制剂要掺入牙科胶粘剂中。

方法

通过比色法检测MMP-8的激活和抑制。对照组，MMP8-I和洗必泰（CHX）组分别存放1天，6个月和1年后，使用微拉伸粘合强度（μTBS）评估粘合强度和破坏模式分布，并使用纳漏分析进行分析。评估较小的散布的银颗粒。

结果

比色分析表明，活化的MMP-8通过粘附程序得以增强，而被MMP8-1或CHX的进一步处理所抑制。与对照组和CHX组相比，MMP8-I组具有明显更高的结合强度，并且经过1年的保存，来自MMP8-I处理的牙本质的杂化层表现出胶原网络的结构完整性，并降低了硝酸银的渗透。

意义

MMP-8抑制I可以防止树脂-牙本质键随时间而降解，这比大规模酶抑制剂CHX更好。它表明MMP8-I可用于牙科领域，以防止胶原蛋白在杂化层中降解，从而延长树脂-牙本质键的寿命。