There is an increasing interest in the effect of dietary polyphenols on the intestinal microbiota and the possible associations between this effect and the development of obesity. However, limited information is available on how these polyphenols affect the gut microbiota and lipid metabolism. The co-action of a high-fat diet (HFD) and tea polyphenol (TP) on gut microbiota and lipid metabolism using a human flora-associated (HFA) C57BL/6J mice model is studied. TP reduced serum total cholesterol, triglyceride, low density lipoprotein, glucose (GLU) and insulin (INS) levels of HFD mice in a dose-dependent manner (P < 0.01). TP also significantly increased acetic acid and butyric acid levels in HFA mice. 16S rRNA V3 region Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) profiles showed that HFD mice had significantly reduced microbial diversity. This reduction could be alleviated by TP, with a significant increase in the richness and diversity of colonic microbiota in the high-fat diet with 0.2% TP (TPM) and high-fat diet with 0.05% TP (TPL) groups (P < 0.05). 454 pyrosequencing analysis showed that the HFD group had a significant increase in the Bacteroidetes to Firmicutes (F/B) ratio (P < 0.001), which could effectively be reversed by TP. The results showed that the changes in composition and diversity of colonic microbiota by TP administration suppressed the host body weight and blood lipid increase in high-fat diet HFA mice. PRACTICAL APPLICATION A high fat diet significantly impacted gut microbiota composition and lipid metabolism in human flora-associated mice, which were largely ameliorated by tea polyphenol (TP). Therefore, TPs may be effectively used in controlling or treating obesity, hyperlipidemia and other related metabolic diseases.

中文翻译：

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绿茶多酚调节高脂饮食处理的 HFA 小鼠的结肠微生物群多样性和脂质代谢

人们越来越关注膳食多酚对肠道微生物群的影响，以及这种影响与肥胖发展之间的可能关联。然而，关于这些多酚如何影响肠道微生物群和脂质代谢的信息有限。使用人类菌群相关 (HFA) C57BL/6J 小鼠模型研究高脂饮食 (HFD) 和茶多酚 (TP) 对肠道微生物群和脂质代谢的共同作用。TP以剂量依赖性方式降低HFD小鼠的血清总胆固醇、甘油三酯、低密度脂蛋白、葡萄糖（GLU）和胰岛素（INS）水平（P<0.01）。TP 还显着增加了 HFA 小鼠的乙酸和丁酸水平。16S rRNA V3 区域聚合酶链反应-变性梯度凝胶电泳 (PCR-DGGE) 图谱显示 HFD 小鼠的微生物多样性显着降低。这种减少可以通过 TP 得到缓解，在 0.2% TP (TPM) 的高脂饮食和 0.05% TP (TPL) 的高脂饮食组中，结肠微生物群的丰富度和多样性显着增加（P < 0.05 ）。454焦磷酸测序分析表明，HFD组的拟杆菌门与厚壁菌门（F/B）比值显着增加（P < 0.001），TP可有效逆转该比例。结果表明，TP给药后结肠微生物群组成和多样性的变化抑制了高脂饮食HFA小鼠的宿主体重和血脂增加。实际应用 高脂肪饮食显着影响人类菌群相关小鼠的肠道微生物群组成和脂质代谢，茶多酚 (TP) 在很大程度上改善了这种情况。因此，TPs可有效用于控制或治疗肥胖、高脂血症及其他相关代谢疾病。