The study of biomolecular interactions is crucial to get more insight into the biological system. The interactions of protein-protein, protein-nucleic acids, protein-sugars, nucleic acid-nucleic acids and protein-small molecules are supporting therapeutics and technological developments. Recently, the development in a large number of analytical techniques for characterizing biomolecular interactions reflect the promising research investments in this field. In this review, microscale thermophoresis technology (MST) is presented as an analytical technique for characterizing biomolecular interactions. Recent years have seen much progress and several applications established. MST is a powerful technique in quantitation of binding events based on the movement of molecules in microscopic temperature gradient. Simplicity, free solutions analysis, low sample volume, short analysis time, and immobilization free are the MST advantages over other competitive techniques. A wide range of studies in biomolecular interactions have been successfully carried out using MST, which tend to the versatility of the technique to use in screening binding events in order to save time, cost and obtained high data quality.

中文翻译：

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用于表征生物分子相互作用的热泳

生物分子相互作用的研究对于深入了解生物系统至关重要。蛋白质-蛋白质、蛋白质-核酸、蛋白质-糖、核酸-核酸和蛋白质-小分子的相互作用正在支持治疗和技术发展。最近，大量用于表征生物分子相互作用的分析技术的发展反映了该领域有希望的研究投资。在这篇综述中，微型热泳技术 (MST) 被介绍为一种表征生物分子相互作用的分析技术。近年来取得了很大进展，并建立了一些应用程序。MST 是一种基于分子在微观温度梯度中运动的结合事件定量分析技术。简单，免费的解决方案分析，样品量少、分析时间短和无需固定是 MST 优于其他竞争技术的优势。已经使用 MST 成功地进行了广泛的生物分子相互作用研究，这倾向于使用该技术的多功能性来筛选结合事件，以节省时间、成本并获得高质量的数据。