Enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins, and glycoproteins in biological samples. While the procedure is routine and straightforward, there are a number of variables (reagent selection, volume measurement, temperature, and time) that if not carefully considered, can affect the test outcome. Herein, we describe the development of microfluidic thread/paper-based analytical devices (µTPAD), microfluidic fabric-based analytical devices (µFAD), and microfluidic thread-based analytical devices (µTAD) as new platforms for ELISA. The quantitative detection of biotinylated goat anti-mouse IgG (system one) and rabbit IgG (system two) antibodies via colorimetric analysis is detailed. We explain the design and fabrication of the devices and the step-by-step protocol for the ELISA. A comparison between the techniques is described and the results obtained from them elucidated.

中文翻译：

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线纸和织物酶联免疫吸附测定 (ELISA)

酶联免疫吸附测定 (ELISA) 是一种免疫学测定，通常用于测量生物样品中的抗体、抗原、蛋白质和糖蛋白。虽然该程序是常规且简单的，但有许多变量（试剂选择、体积测量、温度和时间）如果不仔细考虑，可能会影响测试结果。在此，我们描述了微流控线/纸基分析设备 (µTPAD)、基于微流控织物的分析设备 (µFAD) 和微流控线分析设备 (µTAD) 作为 ELISA 的新平台的发展。详细介绍了生物素化山羊抗小鼠IgG（系统一）和兔IgG（系统二）抗体的比色定量检测。我们解释了设备的设计和制造以及 ELISA 的分步协议。描述了这些技术之间的比较，并阐明了从它们中获得的结果。