Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering†,Lab on a Chip

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Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-02-09 00:00:00 , DOI: 10.1039/c7lc01217a
Lidia Morelli _{1,

2,

3,

4} , Laura Serioli _{1,

2,

3,

4} , Francesca Alessandra Centorbi _{5,

6,

7} , Christian Bille Jendresen _{2,

3,

4,

8} , Marco Matteucci _{1,

2,

3,

4} , Oleksii Ilchenko _{1,

2,

3,

4} , Danilo Demarchi _{5,

6,

7} , Alex Toftgaard Nielsen _{2,

3,

4,

8} , Kinga Zór _{1,

2,

3,

4} , Anja Boisen _{1,

2,

3,

4}

Affiliation

We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid–liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding. The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.

中文翻译：

使用液-液萃取和表面增强拉曼散射技术对转基因大肠杆菌进行筛选的注塑盘上实验室平台†

我们介绍了集成了样品预处理（过滤和液-液萃取）和检测（基于SERS的传感）的自动离心微流控平台的开发。该平台包括八个校准模块和四个测定模块，这些模块通过注塑成型由聚丙烯制成，并通过超声波焊接进行粘合。该平台用于检测细菌上清液中的次级细菌代谢产物（对香豆酸）。所获得的提取效率可与分批实验获得的值相媲美，并且基于SERS的检测结果与HPLC分析显示出良好的相关性。

更新日期：2018-02-09

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