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The effects of surface bioactivity and sustained-release of genistein from a mesoporous magnesium-calcium-silicate/PK composite stimulating cell responses in vitro, and promoting osteogenesis and enhancing osseointegration in vivo†
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-02-09 00:00:00 , DOI: 10.1039/c7bm01017f Liang Cai 1, 2, 3, 4 , Jue Zhang 1, 2, 3, 4 , Jun Qian 1, 2, 3, 4 , Quan Li 4, 5, 6, 7, 8 , Hong Li 4, 9, 10, 11 , Yonggang Yan 4, 9, 10, 11 , Shicheng Wei 12, 13, 14, 15, 16 , Jie Wei 1, 2, 3, 4 , Jiacan Su 4, 5, 6, 7, 8
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-02-09 00:00:00 , DOI: 10.1039/c7bm01017f Liang Cai 1, 2, 3, 4 , Jue Zhang 1, 2, 3, 4 , Jun Qian 1, 2, 3, 4 , Quan Li 4, 5, 6, 7, 8 , Hong Li 4, 9, 10, 11 , Yonggang Yan 4, 9, 10, 11 , Shicheng Wei 12, 13, 14, 15, 16 , Jie Wei 1, 2, 3, 4 , Jiacan Su 4, 5, 6, 7, 8
Affiliation
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The surface of a mesoporous magnesium-calcium-silicate (m-MCS)/polyetheretherketone (PK) composite (MPC) was modified by sand blasting, and genistein (GS) was loaded inside the nanopores of the m-MCS on the modified MPC (MPCm) surface. The results showed that compared with MPC, the surface roughness and hydrophilcity of MPCm obviously improved with more m-MCS exposed on its surface. Moreover, no obvious differences in surface roughness and hydrophilcity were found between MPCm and GS loaded MPCm (MPCm-Ge), and both of them possessed an improved apatite mineralization ability in simulated body fluid solution (SBF) compared with MPC, indicating excellent surface bioactivity. Moreover, the MPCm obviously stimulated the adhesion, proliferation, differentiation and gene expressions of MC3T3-E1 cells compared with MPC, and the sustained-release of GS from the MPCm-Ge surface further significantly promoted the cell proliferation, differentiation and gene expression. According to the Micro-CT, histological and SEM analysis, the results demonstrated that the MPCm obviously improved osteogenesis and enhanced osseointegration in vivo compared with MPC, and the release of GS from the MPCm-Ge surface further significantly improved osteogenesis and enhanced osseointegration. In summary, the significant promotion of cell responses in vitro, and the improvements of osteogenesis and the enhancement of osseointegration in vivo were attributed to the effects of surface bioactivity and GS sustained-release from the MPCm-Ge surface. Therefore, MPCm-Ge would be a potential candidate for orthopedic and dental applications.
中文翻译：
金雀异黄素从中孔镁钙硅酸盐/ PK复合材料的表面生物活性和缓释的影响在体外刺激细胞反应，并在体内促进成骨和增强骨整合†
介孔镁硅酸钙（m-MCS）/聚醚醚酮（PK）复合材料（MPC）的表面通过喷砂处理进行了改性，染料木黄酮（GS）被装载在改性MPC上m-MCS的纳米孔内（ MPCm）表面。结果表明，与MPC相比，MPCm的表面粗糙度和亲水性得到了明显改善，且其表面暴露出更多的m-MCS。此外，MPCm和GS负载的MPCm（MPCm-Ge）在表面粗糙度和亲水性上没有发现明显差异，并且与MPC相比，它们在模拟体液溶液（SBF）中均具有改善的磷灰石矿化能力，表明其具有出色的表面生物活性。 。此外，与MPC相比，MPCm明显刺激了MC3T3-E1细胞的粘附，增殖，分化和基因表达，GS从MPCm-Ge表面的持续释放进一步显着促进了细胞的增殖，分化和基因表达。根据显微CT，组织学和扫描电镜分析，结果表明MPCm明显改善了成骨作用并增强了骨整合在体内与MPC相比，GS从MPCm-Ge表面的释放进一步显着改善了成骨作用并增强了骨整合。综上所述，显著促进细胞反应体外，和成骨的改进和骨结合的增强体内归因于表面活性的影响，GS缓释从MPCM戈表面。因此，MPCm-Ge将成为骨科和牙科应用的潜在候选者。
更新日期：2018-02-09
中文翻译：
金雀异黄素从中孔镁钙硅酸盐/ PK复合材料的表面生物活性和缓释的影响在体外刺激细胞反应，并在体内促进成骨和增强骨整合†
介孔镁硅酸钙（m-MCS）/聚醚醚酮（PK）复合材料（MPC）的表面通过喷砂处理进行了改性，染料木黄酮（GS）被装载在改性MPC上m-MCS的纳米孔内（ MPCm）表面。结果表明，与MPC相比，MPCm的表面粗糙度和亲水性得到了明显改善，且其表面暴露出更多的m-MCS。此外，MPCm和GS负载的MPCm（MPCm-Ge）在表面粗糙度和亲水性上没有发现明显差异，并且与MPC相比，它们在模拟体液溶液（SBF）中均具有改善的磷灰石矿化能力，表明其具有出色的表面生物活性。 。此外，与MPC相比，MPCm明显刺激了MC3T3-E1细胞的粘附，增殖，分化和基因表达，GS从MPCm-Ge表面的持续释放进一步显着促进了细胞的增殖，分化和基因表达。根据显微CT，组织学和扫描电镜分析，结果表明MPCm明显改善了成骨作用并增强了骨整合在体内与MPC相比，GS从MPCm-Ge表面的释放进一步显着改善了成骨作用并增强了骨整合。综上所述，显著促进细胞反应体外，和成骨的改进和骨结合的增强体内归因于表面活性的影响，GS缓释从MPCM戈表面。因此，MPCm-Ge将成为骨科和牙科应用的潜在候选者。
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