A Miniaturized Escherichia coli Green Light Sensor with High Dynamic Range,ChemBioChem

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A Miniaturized Escherichia coli Green Light Sensor with High Dynamic Range
ChemBioChem ( IF 2.6 ) Pub Date : 2018-03-23 , DOI: 10.1002/cbic.201800007
Nicholas T. Ong ₁ , Jeffrey J. Tabor _{1,

2}

Affiliation

Engineering bacterial photoreceptors: We have previously engineered green light sensors from the Synechocystis PCC 6803 CcaSR two‐component system, with six‐ and 117‐fold dynamic range, respectively. Here we have introduced a double PAS domain deletion (recently demonstrated to decrease leakiness) into our system, resulting in a CcaSR system with four times lower leakiness and an almost 600‐fold dynamic range.

中文翻译：

高动态范围的小型化大肠杆菌绿光传感器

工程细菌感光体：我们先前已经工程化的绿色光传感器从集胞藻PCC 6803 CcaSR双组分系统，具有六和117倍的动态范围，分别。在这里，我们在系统中引入了双PAS域删除（最近证明可以减少泄漏），从而使CcaSR系统的泄漏率降低了四倍，动态范围几乎提高了600倍。

更新日期：2018-03-23

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