Simultaneous determination of fifteen toxic alkaloids in meat dishes and vegetable dishes using double layer pipette tip magnetic dispersive solid phase extraction followed by UFLC-MS/MS,Analytical Methods

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Simultaneous determination of fifteen toxic alkaloids in meat dishes and vegetable dishes using double layer pipette tip magnetic dispersive solid phase extraction followed by UFLC-MS/MS
Analytical Methods ( IF 2.7 ) Pub Date : 2018-02-09 00:00:00 , DOI: 10.1039/c7ay02608k
He-Li Cheng _{1,

2,

3,

4} , Feng-Lian Wang _{1,

2,

3,

4} , Yong-Gang Zhao _{1,

2,

3,

4,

5} , Yun Zhang _{1,

2,

3,

4} , Mi-Cong Jin _{4,

5,

6,

7,

8} , Yan Zhu _{1,

2,

3,

4}

Affiliation

A novel double layer pipette tip magnetic dispersive solid phase extraction (DPT-MSPE) based on polyamidoamine-functionalized magnetic carbon nanotubes (PAMAM@Mag-CNTs) was developed for the purification of fifteen toxic alkaloids in meat dishes and vegetable dishes. PAMAM@Mag-CNTs were synthesized through direct reaction between the carboxy group on the surface of the Mag-CNTs and the peripheral amino group of the polyamidoamine (PAMAM) dendrimers. The magnetism of PAMAM@Mag-CNTs simplified the post-processing during preparation of the sorbent, and reinforced the interaction between the sorbents and the analytes during the cleanup procedure. An ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) coupled with DPT-MSPE method has been developed to determine fifteen alkaloids in meat dishes and vegetable dishes. Under the optimal conditions, satisfactory recoveries, i.e., 83.4–125% for meat dishes and 84.2–122% for vegetable dishes, were obtained due to the high cleanup efficiency of the DPT-MSPE. Furthermore, the method, which displays good linearity (r ≥ 0.9991), acceptable precision (RSD < 8%), and low limits of quantification (0.054–1.058 ng g⁻¹), has been successfully applied to several food samples, and it was confirmed that PAMAM@Mag-CNTs DPT-MSPE was a highly effective extraction method for alkaloids in cooked food samples.

中文翻译：

双层移液器吸头磁分散固相萃取-UFLC-MS / MS同时测定肉类和蔬菜类中的十五种有毒生物碱

开发了一种基于聚酰胺胺官能化的磁性碳纳米管（PAMAM @ Mag-CNTs）的新型双层移液器吸头磁分散固相萃取（DPT-MSPE），用于纯化肉类和蔬菜类中的十五种有毒生物碱。PAMAM @ Mag-CNTs是通过Mag-CNTs表面的羧基与聚酰胺酰胺（PAMAM）树枝状聚合物的外围氨基之间的直接反应合成的。PAMAM @ Mag-CNT的磁性简化了吸附剂制备过程中的后处理过程，并增强了清洁过程中吸附剂与分析物之间的相互作用。建立了结合DPT-MSPE方法的超快速液相色谱-串联四极杆质谱（UFLC-MS / MS），用于测定肉类和蔬菜类中的15种生物碱。即，对于肉菜83.4-125％和蔬菜的菜84.2-122％，均由于DPT-MSPE的高效率净化得到。此外，所述方法，其显示出良好的线性关系（ř ≥0.9991），可接受的精确度（RSD <8％），和定量的低限（0.054-1.058纳克克^-1），已经成功地应用到多个食物样本，并且它证实PAMAM @ Mag-CNTs DPT-MSPE是一种高效提取熟食品样品中生物碱的方法。

更新日期：2018-02-09

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