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Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-02-08 00:00:00 , DOI: 10.1039/c7tb02966g M. Kamran Khan 1, 2, 3, 4, 5 , Jianquan Luo 1, 2, 3, 4, 5 , Zhaoshuai Wang 1, 2, 3, 4, 5 , Rashid Khan 1, 2, 3, 4, 5 , Xiangrong Chen 1, 2, 3 , Yinhua Wan 1, 2, 3, 4, 5
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-02-08 00:00:00 , DOI: 10.1039/c7tb02966g M. Kamran Khan 1, 2, 3, 4, 5 , Jianquan Luo 1, 2, 3, 4, 5 , Zhaoshuai Wang 1, 2, 3, 4, 5 , Rashid Khan 1, 2, 3, 4, 5 , Xiangrong Chen 1, 2, 3 , Yinhua Wan 1, 2, 3, 4, 5
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Alginate dialdehyde (ADA), a biocompatible polymer, was used as an intermediate layer on a nylon membrane to readily fabricate cation exchange (CEX), metal-affinity (Me-affinity), histidine-affinity (His-affinity) and peptide-affinity (Pep-affinity) membrane adsorbers without any organic solvent usage. All the membrane adsorbers exhibited a high selectivity in the fractionation of a IgG (immunoglobulin)/HSA (human serum albumin) mixture. Along with a high purity of 100%, a high IgG binding capacity of 30, 24, 21 and 28 mg mL−1 (membrane volume) was achieved by the CEX, Me-affinity, His-affinity and Pep-affinity membrane adsorbers in flow-through mode respectively, which is superior to those of the reported membrane adsorbers. Furthermore, the CEX and Pep-affinity adsorbers were tested for the separation of IgG from human plasma solution. The carboxylic groups along with the peptides on the Pep-affinity adsorber captured IgG synergistically with a higher recovery and purity (99% and 98.6%). This synergetic adsorber showed a qmax and dynamic binding capacity (DBC) of 138.4 and 38.7 mg mL−1 respectively for IgG binding which was somewhat higher than that of the reported protein A agarose (having qmax and DBC of 84 and 24.4 mg g−1 respectively). The present work indicated that the ADA layer not only activated the membrane surface to attach various adsorptive ligands under mild conditions, but also reduced non-specific adsorption. Due to the versatile linking function and green reaction conditions, the ADA coating on the nylon membrane is promising for the preparation of diverse membrane adsorbers.
中文翻译：
海藻酸二醛与尼龙膜相遇：用于膜吸附剂的便捷，绿色制造的多功能平台†
海藻酸二醛（ADA）是一种生物相容性聚合物，被用作尼龙膜上的中间层，可轻松制造阳离子交换（CEX），金属亲和力（Me-亲和力），组氨酸亲和力（His-亲和力）和肽亲和力（Pep-affinity）膜吸附器，无需使用任何有机溶剂。所有膜吸附剂在IgG（免疫球蛋白）/ HSA（人血清白蛋白）混合物的分级分离中均显示出高选择性。伴随着100％的高纯度以及30、24、21和28 mg mL -1的高IgG结合能力（膜体积）分别通过CEX，Me-affinity，His-affinity和Pep-affinity膜吸附器在流通模式下获得，优于已报道的膜吸附器。此外，测试了CEX和Pep亲和吸附剂从人血浆溶液中分离IgG的能力。Pep亲和吸附器上的羧基和肽协同捕获IgG，回收率和纯度更高（分别为99％和98.6％）。该协同吸附剂显示的IgG结合的q max和动态结合容量（DBC）分别为138.4和38.7 mg mL -1，略高于已报道的蛋白A琼脂糖（q max和DBC分别为84和24.4 mg g）。-1分别）。目前的工作表明，ADA层不仅可以在温和的条件下活化膜表面以附着各种吸附性配体，而且还可以减少非特异性吸附。由于通用的连接功能和绿色反应条件，尼龙膜上的ADA涂层有望用于制备各种膜吸附剂。
更新日期：2018-02-08
中文翻译：
海藻酸二醛与尼龙膜相遇：用于膜吸附剂的便捷，绿色制造的多功能平台†
海藻酸二醛（ADA）是一种生物相容性聚合物，被用作尼龙膜上的中间层，可轻松制造阳离子交换（CEX），金属亲和力（Me-亲和力），组氨酸亲和力（His-亲和力）和肽亲和力（Pep-affinity）膜吸附器，无需使用任何有机溶剂。所有膜吸附剂在IgG（免疫球蛋白）/ HSA（人血清白蛋白）混合物的分级分离中均显示出高选择性。伴随着100％的高纯度以及30、24、21和28 mg mL -1的高IgG结合能力（膜体积）分别通过CEX，Me-affinity，His-affinity和Pep-affinity膜吸附器在流通模式下获得，优于已报道的膜吸附器。此外，测试了CEX和Pep亲和吸附剂从人血浆溶液中分离IgG的能力。Pep亲和吸附器上的羧基和肽协同捕获IgG，回收率和纯度更高（分别为99％和98.6％）。该协同吸附剂显示的IgG结合的q max和动态结合容量（DBC）分别为138.4和38.7 mg mL -1，略高于已报道的蛋白A琼脂糖（q max和DBC分别为84和24.4 mg g）。-1分别）。目前的工作表明，ADA层不仅可以在温和的条件下活化膜表面以附着各种吸附性配体，而且还可以减少非特异性吸附。由于通用的连接功能和绿色反应条件，尼龙膜上的ADA涂层有望用于制备各种膜吸附剂。
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