Pulsatile plasma filtration and cell-free DNA amplification using a water-head-driven point-of-care testing chip†,Lab on a Chip

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Pulsatile plasma filtration and cell-free DNA amplification using a water-head-driven point-of-care testing chip†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-02-08 00:00:00 , DOI: 10.1039/c7lc01328k
Yonghun Lee _{1,

2,

3,

4} , Dong-Min Kim _{2,

3,

4,

5} , Zhenglin Li _{1,

2,

3,

4} , Dong-Eun Kim _{2,

3,

4,

5} , Sung-Jin Kim _{1,

2,

3,

4}

Affiliation

We demonstrate a microfiltration chip that separates blood plasma by using water-head-driven pulsatile pressures rather than any external equipment and use it for on-chip amplification of nucleic acids. The chip generates pulsatile pressures to significantly reduce filter clogging without hemolysis, and consists of an oscillator, a plasma-extraction pump, and filter units. The oscillator autonomously converts constant water-head pressure to pulsatile pressure, and the pump uses the pulsatile pressure to extract plasma through the filter. Because the pulsatile pressure can periodically clear blood cells from the filter surface, filter clogging can be effectively reduced. In this way, we achieve plasma extraction with 100% purity and 90% plasma recovery at 15% hematocrit. During a 10 min period, the volume of plasma extracted was 43 μL out of a 243 μL extraction volume at 15% hematocrit. We also studied the influence of the pore size and diameter of the filter, blood loading volume, oscillation period, and hematocrit level on the filtration performance. To demonstrate the utility of our chip for point-of-care testing (POCT) applications, we successfully implemented on-chip amplification of a nucleic acid (miDNA21) in plasma filtered from blood. We expect our chip to be useful not only for POCT applications but also for other bench-top analysis tools using blood plasma.

中文翻译：

使用水头驱动的即时护理测试芯片，实现脉冲血浆过滤和无细胞DNA扩增†

我们演示了一种微滤芯片，该芯片通过使用水头驱动的脉动压力而不是任何外部设备来分离血浆，并将其用于芯片上的核酸扩增。该芯片产生脉动压力，以显着减少过滤器的堵塞而不会发生溶血，它由振荡器，血浆提取泵和过滤器单元组成。振荡器自动将恒定的水头压力转换为脉动压力，泵使用脉动压力通过过滤器提取血浆。因为脉动压力可以周期性地从过滤器表面清除血细胞，所以可以有效地减少过滤器堵塞。这样，我们可以在15％的血细胞比容下实现100％的纯度和90％的血浆回收率的血浆提取。在10分钟内，在15％的血细胞比容下，提取的血浆体积为243μL的提取体积中的43μL。我们还研究了过滤器孔径和直径，血液加载量，振荡周期和血细胞比容水平对过滤性能的影响。为了证明我们的芯片在即时检测（POCT）应用中的实用性，我们成功地在血液中过滤的血浆中实现了核酸（miDNA21）的芯片上扩增。我们希望我们的芯片不仅对POCT应用有用，而且对使用血浆的其他台式分析工具也有用。为了证明我们的芯片在即时检测（POCT）应用中的实用性，我们成功地在血液中过滤的血浆中实现了核酸（miDNA21）的芯片上扩增。我们希望我们的芯片不仅对POCT应用有用，而且对使用血浆的其他台式分析工具也有用。为了证明我们的芯片在即时检测（POCT）应用中的实用性，我们成功地在血液中过滤的血浆中实现了核酸（miDNA21）的芯片上扩增。我们希望我们的芯片不仅对POCT应用有用，而且对使用血浆的其他台式分析工具也有用。

更新日期：2018-02-08

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