A study of the diffusion dynamics and concentration distribution of gold nanospheres (GNSs) without fluorescent labeling inside live cells using fluorescence single particle spectroscopy†,Nanoscale

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A study of the diffusion dynamics and concentration distribution of gold nanospheres (GNSs) without fluorescent labeling inside live cells using fluorescence single particle spectroscopy†
Nanoscale ( IF 5.8 ) Pub Date : 2018-02-08 00:00:00 , DOI: 10.1039/c7nr08722e
Fangchao Liu _{1,

2,

3,

4,

5} , Chaoqing Dong _{1,

2,

3,

4,

5} , Jicun Ren _{1,

2,

3,

4,

5}

Affiliation

Colloidal gold nanospheres (GNSs) have become important nanomaterials in biomedical applications due to their special optical properties, good chemical stability, and biocompatibility. However, measuring the diffusion coefficients or concentration distribution of GNSs within live cells accurately without any extra fluorescent labeling in situ has still not been resolved. In this work, a single particle method is developed to study the concentration distribution of folic acid-modified GNSs (FA-GNSs) internalized via folate receptors, and investigates their diffusion dynamics within live cells using single particle fluorescence correlation spectroscopy (FCS). We optimized the experimental conditions and verified the feasibility of 30 nm GNSs without extra fluorescence labeling being used for single particle detection inside live cells. Then, the FCS characterization strategy was used to measure the concentration and diffusion coefficient distributions of GNSs inside live cells and the obtained results were basically in agreement with those obtained by TEM. The results demonstrate that our strategy is characterized as an in situ, nondestructive, rapid and dynamic method for the assay of live cells, and it may be widely used in the further design of GNP-based drug delivery and therapeutics.

中文翻译：

使用荧光单粒子光谱法研究活细胞内部没有荧光标记的金纳米球（GNS）的扩散动力学和浓度分布†

胶体金纳米球（GNS）由于其特殊的光学性能，良好的化学稳定性和生物相容性，已成为生物医学应用中重要的纳米材料。然而，测量的全球导航卫星系统的活细胞内的扩散系数或浓度分布准确而无需任何额外的荧光标记原位仍然没有得到解决。在这项工作中，单个颗粒方法用于研究内化叶酸-经修改的GNSS（FA-GNSS）的浓度分布通过叶酸受体，并使用单粒子荧光相关光谱（FCS）研究它们在活细胞内的扩散动力学。我们优化了实验条件并验证了30 nm GNS的可行性，而无需使用额外的荧光标记将其用于活细胞内部的单颗粒检测。然后，采用FCS表征策略测量活细胞内GNS的浓度和扩散系数分布，所得结果与TEM获得的结果基本一致。结果表明，我们的策略是一种原位，无损，快速和动态的活细胞分析方法，可广泛用于基于GNP的药物递送和治疗方法的进一步设计。

更新日期：2018-02-08

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