Understanding the influence of various physical cues on cell behavior is critical for the design of bioactive biomaterials optimized for tissue regeneration. Hydrogels provide microenvironments suitable for cells and tissues, but the introduction of tunable physical cues in hydrogels is often challenging. Here, silk nanofiber hydrogels with tunable, aligned structures were prepared under electric fields and used to influence cell behavior. Cell responses to the aligned structures were qualitatively and quantitatively evaluated by investigating cell proliferation, morphology, and migration behavior when compared to cells cultured on silk nanofiber hydrogels with homogeneous structures as controls. The cells seeded on anisotropic hydrogels showed oriented morphology, suggesting the link between aligned physical cues on cell morphology. The results indicated that tunable, aligned structures in hydrogels are effective for the design of bioactive biomaterials with preferred functions.

中文翻译：

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可调各向异性结构的纳米丝水凝胶对细胞行为的控制

理解各种物理线索对细胞行为的影响对于优化组织再生的生物活性生物材料的设计至关重要。水凝胶提供适用于细胞和组织的微环境，但是在水凝胶中引入可调节的物理线索通常具有挑战性。在这里，在电场下制备了具有可调的，排列的结构的丝纳米纤维水凝胶，并用于影响细胞行为。当与在均质结构的丝绸纳米纤维水凝胶上培养的细胞进行比较时，通过研究细胞增殖，形态和迁移行为，定性和定量评估了对对齐结构的细胞反应。接种在各向异性水凝胶上的细胞表现出定向的形态，表明排列的物理线索之间在细胞形态上的联系。