In this article, we review the application of fluorescence correlation spectroscopy (FCS) methods to studies on live cells. We begin with a brief overview of the theory underlying FCS, highlighting the type of information obtainable. We then focus on circular scanning FCS. Specifically, we discuss instrumentation and data analysis and offer some considerations regarding sample preparation. Two examples from the literature are discussed in detail. First, we show how this method, coupled with the photon counting histogram analysis, can provide information on yeast ribosomal structures in live cells. The combination of scanning FCS with dual channel detection in the study of lipid domains in live cells is also illustrated.

中文翻译：

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扫描荧光相关光谱全面发展

在本文中，我们回顾了荧光相关光谱 (FCS) 方法在活细胞研究中的应用。我们首先简要概述 FCS 的基础理论，重点介绍可获得的信息类型。然后我们专注于圆形扫描 FCS。具体来说，我们讨论仪器和数据分析，并提供一些有关样品制备的注意事项。详细讨论了文献中的两个例子。首先，我们展示了这种方法如何与光子计数直方图分析相结合，可以提供有关活细胞中酵母核糖体结构的信息。还说明了扫描 FCS 与双通道检测在活细胞脂质域研究中的结合。