Role of topological scale in the differential fouling of Pseudomonas aeruginosa and Staphylococcus aureus bacterial cells on wrinkled gold-coated polystyrene surfaces†,Nanoscale

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Role of topological scale in the differential fouling of Pseudomonas aeruginosa and Staphylococcus aureus bacterial cells on wrinkled gold-coated polystyrene surfaces†
Nanoscale ( IF 5.8 ) Pub Date : 2018-02-07 00:00:00 , DOI: 10.1039/c7nr08178b
Duy H. K. Nguyen _{1,

2,

3,

4,

5} , Vy T. H. Pham _{1,

2,

3,

4,

5} , Vi Khanh Truong _{1,

2,

3,

4,

5} , Igor Sbarski _{2,

3,

4,

5,

6} , James Wang _{2,

3,

4,

5,

6} , Armandas Balčytis _{1,

2,

3,

4,

5} , Saulius Juodkazis _{1,

2,

3,

4,

5} , David E. Mainwaring _{1,

2,

3,

4,

5} , Russell J. Crawford _{1,

7,

8,

9,

10} , Elena P. Ivanova _{1,

2,

3,

4,

5}

Affiliation

Wrinkled patterns, which possess an extensive surface area over a limited planar space, can provide surface features ranging across the nano- and microscale that have become an engineering material with the flexibility to be tuneable for a number of technologies. Here, we investigate the surface parameters that influence the attachment response of two model bacteria (P. aeruginosa and S. aureus) to wrinkled gold-coated polystyrene surfaces having topologies at the nano- and microscale. Together with flat gold films as the controls, surface feature heights spanned 2 orders of magnitude (15 nm, 200 nm, and 1 micron). The surface wrinkle topology was shown through confocal laser scanning microscopic, atomic force microscopic and scanning electron microscopic image analyses to consist of air–water interfacial areas unavailable for bacterial attachment, which were also shown to be stable by time-lapsed contact angle measurements. Imposition of the nanoscale wrinkles reduced P. aeruginosa attachment to 57% and S. aureus attachment to 20% of their flat equivalent surfaces whereas wrinkles at the microscale further reduced these attachments to 7.5% and 14.5%, respectively. The density of attachments indicated an inherent species specific selectivity that changed with feature dimension, attributable to the scale of the air–water interfaces in contact with the bacterial cell. Parameters influencing static bacterial attachment were the total projected surface areas minus the air–water interface areas and the scale of these respective air–water interfaces (area distribution) with respect to the cell morphology. The range of these controlling parameters may provide new design principles for the evolving suite of physical anti-biofouling materials not reliant on biocidal agents under development.

中文翻译：

拓扑规模在皱纹镀金聚苯乙烯表面上的铜绿假单胞菌和金黄色葡萄球菌细菌细胞的差异结垢中的作用†

起皱的图案在有限的平面空间中具有较大的表面积，可以提供横跨纳米级和微米级范围的表面特征，这些表面特征已成为工程材料，并具有可针对多种技术进行调整的灵活性。在这里，我们调查影响两个模型细菌（铜绿假单胞菌和金黄色葡萄球菌）的附着反应的表面参数）到具有纳米和微米级拓扑结构的起皱的金涂层的聚苯乙烯表面。与平坦的金膜作为对照，表面特征高度跨越2个数量级（15 nm，200 nm和1微米）。通过共聚焦激光扫描显微镜，原子力显微镜和扫描电子显微镜图像分析显示出表面皱纹拓扑结构，其由不可用于细菌附着的空气-水界面区域组成，并且通过延时接触角测量也显示出该表面区域是稳定的。施加纳米级皱纹将铜绿假单胞菌和金黄色葡萄球菌的附着降低至57％附着到其平面等效表面的20％，而微尺度的皱纹进一步将这些附着分别减少到7.5％和14.5％。附着的密度表明固有的物种特异性选择性随特征尺寸而变化，这归因于与细菌细胞接触的空气-水界面的规模。影响静态细菌附着的参数是总投影表面积减去空气-水界面面积，以及这些相对于细胞形态的空气-水界面的比例（面积分布）。这些控制参数的范围可以为不依赖于正在开发的杀生物剂的不断发展的物理防污垢材料套件提供新的设计原理。

更新日期：2018-02-07

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