Zika Immunoassay Based on Surface-Enhanced Raman Scattering Nanoprobes,ACS Sensors

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Zika Immunoassay Based on Surface-Enhanced Raman Scattering Nanoprobes
ACS Sensors ( IF 8.2 ) Pub Date : 2018-02-07 00:00:00 , DOI: 10.1021/acssensors.7b00639
Sabrina A. Camacho _{1,

2} , Regivaldo Gomes Sobral-Filho ₂ , Pedro Henrique B. Aoki _{2,

3} , Carlos José L. Constantino ₁ , Alexandre G. Brolo ₂

Affiliation

Zika virus (ZIKV) was considered a public health emergency of international concern after the 2015 outbreak. Serological tests based on immunoassay platforms is one of the methods applied on the diagnosis of ZIKV and Dengue virus (DENV). However, the high limits of detection (LOD) and the cross-reactivity between ZIKV and DENV are still limitations in immunological tests. In order to tackle these issues, we have designed an immune-specific assay based on surface-enhanced Raman scattering (SERS) nanoprobes. Gold shell-isolated nanoparticles (Au-SHINs) were synthesized with 100 nm Au nanoparticles and 4 nm silica shell thickness coated with Nile Blue (Raman reporter). Then, the SERS nanoprobes were wrapped in a final silica shell and functionalized with monoclonal anti-ZIKV NS1 antibodies. Concentrations of ZIKV NS1 down to 10 ng/mL were probed free of cross-reactivity with DENV NS1 antigens.

中文翻译：

基于表面增强拉曼散射纳米探针的Zika免疫测定

2015年爆发后，寨卡病毒（ZIKV）被认为是国际关注的突发公共卫生事件。基于免疫测定平台的血清学检测是诊断ZIKV和登革热病毒（DENV）的方法之一。但是，高检测限（LOD）和ZIKV与DENV之间的交叉反应仍然是免疫学测试的局限性。为了解决这些问题，我们设计了一种基于表面增强拉曼散射（SERS）纳米探针的免疫特异性测定法。合成了金壳分离的纳米颗粒（Au-SHINs），其中包含100 nm的Au纳米颗粒和4 nm的二氧化硅壳厚度，涂有尼罗河蓝（拉曼报道分子）。然后，将SERS纳米探针包裹在最终的硅胶外壳中，并用单克隆抗ZIKV NS1抗体进行功能化。

更新日期：2018-02-07

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