Two-Phase Flow in Liquid Chromatography, Part 1: Experimental Investigation and Theoretical Description,Industrial & Engineering Chemistry Research

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Two-Phase Flow in Liquid Chromatography, Part 1: Experimental Investigation and Theoretical Description
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2018-02-21 00:00:00 , DOI: 10.1021/acs.iecr.7b05153
Franziska Ortner ₁ , Marco Mazzotti ₁

Affiliation

Two-phase flow is investigated in the context of liquid chromatography. In order to describe the column behavior in the presence of multiple convective phases, an equilibrium theory model is established, accounting for both adsorption and multiphase flow. The validity of this model is studied specifically for the experimental system phenetole–methanol–water on a Zorbax 300SB-C18 column. For this purpose, the experimental system is characterized in terms of thermodynamic liquid–liquid equilibria, adsorption behavior, and hydrodynamic properties by independent experimental campaigns. Mathematical relationships are established, describing the investigated effects. Implementation of these relationships in the equilibrium theory model and application of the model to calculate elution profiles provides a quantitative description of experimental data from dynamic column experiments. The good agreement validates the model, its assumptions, and the established relationships. This study provides a thorough insight on the implications of multiple convective phases in the context of liquid chromatography.

中文翻译：

液相色谱两相流，第1部分：实验研究和理论描述

在液相色谱法中研究了两相流。为了描述存在多个对流相时的色谱柱行为，建立了一个平衡理论模型，该模型同时考虑了吸附和多相流动。该模型的有效性在Zorbax 300SB-C18色谱柱上针对苯酚-甲醇-水的实验系统进行了专门研究。为此，通过独立的实验活动，以热力学的液-液平衡，吸附行为和流体力学特性为特征对实验系统进行了表征。建立数学关系，描述所研究的效果。这些关系在平衡理论模型中的实现以及该模型在计算洗脱曲线中的应用提供了对来自动态柱实验的实验数据的定量描述。良好的协议可以验证模型，其假设以及已建立的关系。这项研究对液相色谱中多个对流相的影响提供了透彻的见解。

更新日期：2018-02-21

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