Thin films based on chitosan, collagen, and glycosaminoglycans isolated from fish skin were obtained by solvent evaporation. The films were characterized by different analyses, e.g. surface free energy determination, swelling tests, roughness, mechanical and thermal measurements. Moreover, the degradation studies were carried out by the film treatment with collagenase. The results showed that the properties of the films based on chitosan and collagen can be modified by the glycosaminoglycans addition. It was noticed that the addition of glycosaminoglycans enhances the surface hydrophilicity and reduces surface free energy. Surfaces of films modified by glycosaminoglycans (GAGs) show more roughness which inhibits the risk of biofilm formation. The highest films swelling was obtained after 2 h immersion in phosphate-buffered saline (PBS). After their immersion in PBS, the films were more elastic, which was assumed on the basis of the elongation at break values higher than in the case of films on a dry surface. The proposed films can create biocompatible coatings for biomedical applications.

通过溶剂蒸发获得了基于从鱼皮中分离的壳聚糖，胶原蛋白和糖胺聚糖的薄膜。通过不同的分析对薄膜进行表征，例如表面自由能测定，溶胀测试，粗糙度，机械和热测量。此外，通过用胶原酶进行膜处理来进行降解研究。结果表明，基于壳聚糖和胶原蛋白的薄膜的性能可以通过添加糖胺聚糖来改变。注意到糖胺聚糖的添加增强了表面亲水性并降低了表面自由能。糖胺聚糖（GAG）修饰的薄膜表面表现出更大的粗糙度，从而抑制了生物膜形成的风险。浸入磷酸盐缓冲盐水（PBS）2小时后，可获得最高的膜溶胀度。将其浸入PBS后，薄膜具有更大的弹性，这是基于断裂伸长率值得出的，该值高于干表面上的薄膜。所提出的膜可以产生用于生物医学应用的生物相容性涂层。