Multiphoton microscopy (MPM) has been widely used for three-dimensional biological imaging. Here, based on the photon-induced charge state conversion process, we demonstrated a low-power high-resolution MPM with a nitrogen vacancy (NV) center in diamond. Continuous wave green and orange lasers were used to pump and detect the two-photon charge state conversion, respectively. The power of the laser for multiphoton excitation was 40 μW. Both the axial and lateral resolutions were improved approximately 1.5 times compared with confocal microscopy. The results can be used to improve the resolution of the NV center-based quantum sensing and biological imaging.

中文翻译：

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具有低功率连续波激光泵的高分辨率多光子显微镜

多光子显微镜（MPM）已被广泛用于三维生物成像。在这里，基于光子诱导的电荷状态转换过程，我们展示了低功率高分辨率MPM，其金刚石中的氮空位（NV）中心。连续波绿色和橙色激光分别用于泵浦和检测双光子电荷状态转换。用于多光子激发的激光功率为40μW。与共聚焦显微镜相比，轴向和横向分辨率均提高了约1.5倍。结果可用于提高基于NV中心的量子感测和生物成像的分辨率。