A dual-wavelength digital holographic phase and fluorescence microscopy system is demonstrated as the decoding and detection platform of an optical thickness encoded suspension array. The phase imaging path is designed to decode optical thickness encoded microcarriers, and the fluorescence imaging path is used to detect the quantitative information of the bound target analytes. The encoding capacity could be more than 100. The decoding reliability of the phase imaging path is verified by a multiplexed immunoassay experiment. The ability for quantitative analysis of the fluorescence imaging path is confirmed by concentration gradient experiments. This method offers high decoding accuracy and high detection sensitivity of label signals.

中文翻译：

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光学厚度编码悬浮阵列的双波长数字全息相和荧光显微镜

演示了双波长数字全息相位和荧光显微镜系统作为光学厚度编码悬浮阵列的解码和检测平台。相位成像路径设计为对光学厚度编码的微载体进行解码，荧光成像路径用于检测结合的目标分析物的定量信息。编码容量可以超过100。通过多重免疫分析实验验证了相位成像路径的解码可靠性。通过浓度梯度实验确认了荧光成像路径的定量分析能力。该方法提供了较高的解码精度和较高的标签信号检测灵敏度。