Voltage-dependent calcium channels (VDCC) have been shown to regulate neuronal excitability and their antagonists have been used clinically for the control of seizures. While functional studies of VDCC in epileptogenesis in the CA1 area of hippocampus or the dentate gyrus have been done, few studies were carried out in the CA3 area. Given the bursting characteristics of the CA3 neurons, we speculated that VDCC in the CA3 area might play an important role in the epileptogenesis. In the present study in the mouse pilocarpine model of temporal lobe epilepsy, we investigated the alterations of alpha 1 subunits of L-type VDCC in the CA3 area of the hippocampus at different stages of epileptogenesis, i.e., acute stage from 10 min to 1 day during and after pilocapine-induced status epilepticus (SE), latent period at 1 week, and chronic stage with spontaneous recurrent seizures at 2 months after SE. We found that an immediate redistribution of alpha 1 subunits in the CA3 area occurred during SE which might be involved in the seizure occurrence indicated by the Racine score record. Alterations of alpha 1 subunits were also demonstrated in the latent period and chronic stage, which might be related to the epileptogenesis and occurrence of epilepsy. Ca_v1.3, but not Ca_v1.2, was expressed in reactive astrocytes of the CA3 area, indicating the involvement of Ca_v1.3 in the modulation of astrocytic Ca²⁺ homeostasis during epileptogenesis.

电压依赖性钙通道（VDCC）已显示出调节神经元兴奋性的作用，其拮抗剂已在临床上用于控制癫痫发作。尽管已经完成了VDCC在海马或齿状回CA1区癫痫发生中的功能研究，但在CA3区进行的研究很少。考虑到CA3神经元的爆发特征，我们推测CA3区域中的VDCC可能在癫痫发生中起重要作用。在本研究中，小鼠颞叶癫痫的毛果芸香碱模型中，我们研究了在癫痫发生的不同阶段（即急性阶段，从10分钟到1天），海马CA3区L型VDCC的α1亚基的变化在pilocapine诱发的癫痫持续状态（SE）期间和之后，潜伏期为1周，并在SE后2个月出现自发性复发性癫痫发作的慢性阶段。我们发现SE期间发生了CA3区域中α1亚基的立即重新分布，这可能与Racine得分记录所指示的癫痫发作有关。在潜伏期和慢性期也证实了α1亚基的改变，这可能与癫痫的发生和癫痫的发生有关。钙在CA3区的星形胶质细胞中表达的是_v 1.3，而不是Ca _v 1.2，这表明Ca _v 1.3参与了癫痫发生过程中星形细胞Ca ²⁺稳态的调节。