Boronic acid affinity chromatography (BAAC) is one of the most significant methods in glycoproteomics research due to its low bias towards glycopeptides and easy enrichment process. In this work, core-shell structured magnetic metal-organic framework (MOF) composites with abundant boronic acid groups were designed and synthesized for selective glycopeptide enrichment based on BAAC. The as-prepared core-shell structured magnetic MOF composites (denoted as Fe₃O₄@PVP/[email protected] (B)) inherited strong magnetic responsiveness from the Fe₃O₄ core as well as ultrahigh surface area and abundant boronic acid sites from the MOF shell. The affinity between boronic acid and cis-diols groups endowed the composites with improved sensitivity (0.5 fmol/μL) and selectivity (1:100) towards glycopeptides, achieving remarkable results in glycopeptides detection from standard glycoprotein digests as well as complex bio-samples. As a result, a total of 209 N-glycosylation peptides from 89 different glycoproteins were identified from human serum digests, indicating its broad prospect in glycoproteome study.

硼酸亲和色谱法（BAAC）是糖蛋白组学研究中最重要的方法之一，因为它对糖肽的偏倚低并且易于富集。在这项工作中，设计并合成了具有丰富硼酸基团的核-壳结构磁性金属-有机骨架（MOF）复合材料，用于基于BAAC的选择性糖肽富集。制备的核-壳结构磁性MOF复合材料（表示为Fe ₃ O ₄ @ PVP / [电子邮件保护]（B））继承了Fe ₃ O ₄核的强磁响应性，超高表面积和丰富的硼酸。 MOF外壳程序中的网站。硼酸与顺式之间的亲和力-二醇基团使复合物对糖肽具有更高的灵敏度（0.5 fmol /μL）和选择性（1：100），在从标准糖蛋白消化物和复杂生物样品中检测糖肽方面取得了显着结果。结果，从人血清消化物中鉴定出来自89种不同糖蛋白的209种N-糖基化肽，表明其在糖蛋白组学研究中具有广阔的前景。