An innovative electrochemical sensor was fabricated for the sensitive and selective determination of tinidazole (TNZ), based on a carbon paste electrode (CPE) modified with multi-walled carbon nanotubes (MWCNTs) and boron-embedded molecularly imprinted composite membranes (B-MICMs). Density functional theory (DFT) calculations were carried out to investigate the utility of template-monomer interactions to screen appropriate monomers for the rational design of B-MICMs. The distinct synergic effect of MWCNTs and B-MICMs was evidenced by the positive shift of the reduction peak potential of TNZ at B-MICMs/MWCNTs modified CPE (B-MICMs/MWCNTs/CPE) by about 200 mV, and the 12-fold amplification of the peak current, compared with a bare carbon paste electrode (CPE). Moreover, the coordinate interactions between trisubstituted boron atoms embedded in B-MICMs matrix and nitrogen atoms of TNZ endow the sensor with advanced affinity and specific directionality. Thereafter, a highly sensitive electrochemical analytical method for TNZ was established by different pulse voltammetry (DPV) at B-MICMs/MWCNTs/CPE with a lower detection limit (1.25 × 10⁻¹² mol L⁻¹) (S/N = 3). The practical application of the sensor was demonstrated by determining TNZ in pharmaceutical and biological samples with good precision (RSD 1.36% to 3.85%) and acceptable recoveries (82.40%–104.0%).

基于多壁碳纳米管（MWCNT）和硼嵌入分子印迹复合膜（B-MICMs）修饰的碳糊电极（CPE），制造了用于灵敏和选择性测定替硝唑（TNZ）的创新型电化学传感器。 。进行了密度泛函理论（DFT）计算，以研究模板-单体相互作用筛选B-MICM合理设计所需单体的实用性。MWCNTs和B-MICMs的独特协同作用由BNZICMs / MWCNTs修饰的CPE（B-MICMs / MWCNTs / CPE）上TNZ的还原峰电位正移约200 mV证实了12倍与裸碳糊电极（CPE）相比，峰值电流的放大率更高。而且，B-MICMs基质中嵌入的三取代硼原子与TNZ的氮原子之间的坐标相互作用赋予了传感器更高的亲和力和特定的方向性。此后，通过不同的脉冲伏安法（DPV）在B-MICMs / MWCNTs / CPE上建立了对TNZ的高灵敏度电化学分析方法，其检测限较低（1.25×10^-12  mol L ^-1）（S / N = 3）。通过以良好的精密度（RSD为1.36％至3.85％）和可接受的回收率（82.40％–104.0％）测定药物和生物样品中的TNZ，证明了传感器的实际应用。