Very long chain fatty acids (VLCFAs) were identified in four strains of the green alga Botryococcus braunii (Trebouxiophyceae). The algae contained a series of monoenoic fatty acids up to triacontenoic acid and further VLCFAs in amounts around 1% of total fatty acids. The separation of lipid classes using hydrophilic interaction chromatography revealed that the most abundant VLCFAs (28:2, 28:1 and 28:0) were contained in neutral lipids (triacylglycerols and/or diacylglycerols) and in phospholipids (phosphatidic acid and/or phosphatidylcholine). Using non-aqueous reversed-phase liquid chromatography tandem mass spectrometry (NARP-LC/MS2) of the appropriate collected fractions, molecular species of triacylglycerols containing one or two VLCFAs were described and phosphatidylcholines containing VLCFAs were separated for the first time. Because the presence of Botryosphaerella sudetica (Chlorophyceae) as contaminant of Botryococcus braunii strain Droop 1950/807-1 placed some doubts on the results of previous studies, a strain of this green alga of was also analyzed. In contrast to Botryococcus, C16, a substantially lower proportion of C18 polyunsaturated fatty acids and no VLCFAs were detected in Botryosphaerella.

中文翻译：

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葡萄球菌（Trebouxiophyceae、Chlorophyta）的脂质组学分析 - 通过离线二维 LC-串联 MS 鉴定含有极长链脂肪酸的脂质类别

在四种绿藻 Botryococcus braunii (Trebouxiophyceae) 菌株中鉴定出极长链脂肪酸 (VLCFA)。藻类含有一系列单烯脂肪酸，直至三十烯酸，以及占总脂肪酸约 1% 的其他 VLCFA。使用亲水相互作用色谱分离脂质类别表明，最丰富的 VLCFA（28:2、28:1 和 28:0）包含在中性脂质（三酰甘油和/或二酰甘油）和磷脂（磷脂酸和/或磷脂酰胆碱）中）。使用适当收集馏分的非水反相液相色谱串联质谱 (NARP-LC/MS2)，描述了含有一种或两种 VLCFA 的三酰基甘油的分子种类，并首次分离了含有 VLCFA 的磷脂酰胆碱。由于 Botryosphaerella sudetica (Chlorophyceae) 作为 Botryococcus braunii 菌株 Droop 1950/807-1 的污染物的存在，对先前研究的结果提出了一些怀疑，因此还分析了这种绿藻的菌株。与葡萄球菌、C16 相比，在葡萄球菌中检测到 C18 多不饱和脂肪酸的比例要低得多，并且未检测到 VLCFA。