Seven previously undescribed steroidal glycosides, along with three known congeners were isolated from the unripe berries of Solanum nigrum L. (Solanaceae). Their structures were elucidated on basis of 1D and 2D NMR, HR-ESI-MS spectroscopic data and GC analysis after acid hydrolysis. The potential inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide in RAW 264.7 cell line and the anti-proliferative activities against five cancer cell lines (HL-60, U-937, Jurkat, K562 and HepG2) were evaluated. Seven compounds exhibited inhibition activities on NO production with IC50 values ranging from 11.33 to 49.35 μM. Structure-activity relationships of the isolated compounds were also discussed.

中文翻译：

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来自 Solanu m nigru m L.（欧洲黑茄属植物）浆果的抗炎甾体糖苷

从 Solanum nigrum L.（茄科）的未成熟浆果中分离出 7 种以前未描述的甾体糖苷以及 3 种已知的同源物。基于一维和二维核磁共振、HR-ESI-MS光谱数据和酸水解后的GC分析阐明了它们的结构。评估了对 RAW 264.7 细胞系中脂多糖诱导的一氧化氮 (NO) 产生的潜在抑制作用以及对五种癌细胞系（HL-60、U-937、Jurkat、K562 和 HepG2）的抗增殖活性。七种化合物表现出对 NO 产生的抑制活性，IC50 值范围为 11.33 至 49.35 μM。还讨论了分离化合物的构效关系。