Mycotoxins produced by different species of fungi may coexist in cereals and feedstuffs, and could be highly toxic for humans and animals. For quantification of multiple mycotoxins in cereals, we developed a paper-based mycotoxin immune-affinity array. First, paper-based microzone arrays were fabricated by photolithography. Then, monoclonal mycotoxin antibodies were added in a copolymerization reaction with a cross-linker to form an immune-affinity monolith on the paper-based microzone array. With use of a competitive immune-response format, paper-based mycotoxin immune-affinity arrays were successfully applied to detect mycotoxins in samples. The detection limits for deoxynivalenol, zearalenone, T-2 toxin, and HT-2 toxin were 62.7, 10.8, 0.36, and 0.23 μg·kg^-1, respectively, which meet relevant requirements for these compounds in food. The recovery rates were 81–86% for deoxynivalenol, 89–117% for zearalenone, 79–86% for T-2 toxin, and 78–83% for HT-2 toxin, and showed the paper-based immune-affinity arrays had good reproducibility. In summary, the paper-based mycotoxin immune-affinity array provides a sensitive, rapid, accurate, stable, and convenient platform for detection of multiple mycotoxins in agro-foods.

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由不同种类的真菌产生的霉菌毒素可能共存于谷物和饲料中，对人类和动物有剧毒。为了定量分析谷物中多种霉菌毒素，我们开发了一种基于纸质的霉菌毒素免疫亲和阵列。首先，通过光刻制造纸基微区阵列。然后，在与交联剂的共聚反应中添加单克隆真菌毒素抗体，以在纸基微区阵列上形成免疫亲和整体。通过使用竞争性免疫反应形式，基于纸质的霉菌毒素免疫亲和力阵列已成功应用于检测样品中的霉菌毒素。脱氧雪腐烯醇，玉米赤霉烯酮，T-2毒素和HT-2毒素的检出限分别为62.7、10.8、0.36和0.23μg·kg ^-1分别满足食品中这些化合物的相关要求。脱氧雪腐烯醇的回收率为81–86％，玉米赤霉烯酮的回收率为89–117％，T-2毒素的回收率为79–86％，HT-2毒素的回收率为78–83％，表明纸质免疫亲和阵列具有重现性好。总之，基于纸质的霉菌毒素免疫亲和力阵列为检测农产品中的多种霉菌毒素提供了灵敏，快速，准确，稳定和方便的平台。

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