Prunus genus fruit seeds are sources of highly angiotensin-I-converting enzyme (ACE)‐inhibitory peptides. The presence of peptides IYSPH, IYTPH, IFSPR, and VAIP seems to be related to this activity but no previous work has demonstrated the direct relationship between the concentration of these peptides and the antihypertensive activity of hydrolysates. This work describes the development of a method for the quantification of these peptides in Prunus seeds hydrolysates based on capillary liquid chromatography‐IT-MS/MS. The analytical characteristics of the method were evaluated through the study of the linearity, LOD, LOQ, presence of matrix interferences, precision, and recovery. The developed methodology was applied to the determination of the four peptides in seed hydrolysates from different Prunus genus fruits: peaches (7 varieties), plums (2 varieties), nectarines (3 varieties), apricots (2 varieties), cherry, and paraguayo. Peaches and plums seed hydrolysates yielded the highest concentrations of these peptides while paraguayo one showed the lowest concentrations. A high correlation between peptides concentrations was demonstrated suggesting that the four peptides could be released from the same seed proteins.

李属果实种子是高度血管紧张素-I转换酶（ACE）抑制肽的来源。肽IYSPH，IYTPH，IFSPR和VAIP的存在似乎与这种活性有关，但以前的工作尚未证明这些肽的浓度与水解产物的降压活性之间存在直接关系。这项工作描述了基于毛细管液相色谱-IT-MS / MS的李子种子水解产物中这些肽的定量方法的开发。通过研究线性，LOD，LOQ，基质干扰的存在，精密度和回收率，评估了该方法的分析特性。所开发的方法用于测定来自不同种子的种子水解物中的四种肽李属水果：桃（7个品种），李子（2个品种），油桃（3个品种），杏（2个品种），樱桃和巴拉圭。桃子和李子种子水解物产生这些肽的最高浓度，而巴拉圭人显示出最低的浓度。肽浓度之间的高度相关性表明，这四种肽可以从相同的种子蛋白中释放出来。