Comparative Proteomic Analysis of Exosomes and Microvesicles in Human Saliva for Lung Cancer,Journal of Proteome Research

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Comparative Proteomic Analysis of Exosomes and Microvesicles in Human Saliva for Lung Cancer
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-02-13 00:00:00 , DOI: 10.1021/acs.jproteome.7b00770
Yan Sun ₁ , Chunhui Huo ₁ , Zhi Qiao ₁ , Zhi Shang ₁ , Asad Uzzaman ₁ , Sha Liu ₁ , Xiaoteng Jiang ₁ , Liu-Yin Fan ₁ , Liyun Ji ₁ , Xin Guan ₂ , Cheng-Xi Cao ₁ , Hua Xiao ₁

Affiliation

Extracellular vesicles (EVs) are cell-derived microparticles present in most body fluids, mainly including microvesicles and exosomes. EV-harbored proteins have emerged as novel biomarkers for the diagnosis and prediction of different cancers. We successfully isolated microvesicles and exosomes from human saliva, which were further characterized comprehensively. Salivary EV protein profiling in normal subjects and lung cancer patients was systematically compared through utilizing LC–MS/MS-based label-free quantification. 785 and 910 proteins were identified from salivary exosomes and microvesicles, respectively. According to statistical analysis, 150 and 243 proteins were revealed as dysregulated candidates in exosomes and microvesicles for lung cancer. Among them, 25 and 40 proteins originally from distal organ cells were found in the salivary exosomes and microvesicles of lung cancer patients. In particular, 5 out of 25 and 9 out of 40 are lung-related proteins. Six potential candidates were selected for verification by Western blot, and four of them, namely, BPIFA1, CRNN, MUC5B, and IQGAP, were confirmed either in salivary microvesicles or in exosomes. Our data collectively demonstrate that salivary EVs harbor informative proteins that might be used for the detection of lung cancer through a noninvasive way.

中文翻译：

唾液中外泌体和微泡对肺癌的蛋白质组学比较分析

细胞外囊泡（EVs）是大多数体液中存在的细胞来源的微粒，主要包括微囊泡和外泌体。携带EV的蛋白质已成为诊断和预测不同癌症的新型生物标志物。我们成功地从人唾液中分离了微泡和外泌体，对其进行了进一步的全面表征。利用基于LC-MS / MS的无标记定量方法，系统地比较了正常受试者和肺癌患者的唾液EV蛋白谱。分别从唾液外泌体和微泡中鉴定出785和910蛋白。根据统计分析，在肺癌的囊泡和微囊泡中发现150和243个蛋白是失调的候选蛋白。其中，在肺癌患者的唾液外泌体和微泡中发现了来自远端器官细胞的25和40种蛋白质。特别地，25个中的5个和40个中的9个是与肺相关的蛋白质。选择了六种可能的候选蛋白进行Western印迹验证，并在唾液微泡或外泌体中确认了其中的四种，即BPIFA1，CRNN，MUC5B和IQGAP。我们的数据共同证明，唾液电动汽车具有信息蛋白，可通过无创方式用于检测肺癌。在唾液微泡或外泌体中被证实。我们的数据共同证明，唾液电动汽车具有信息蛋白，可通过无创方式用于检测肺癌。在唾液微泡或外泌体中被证实。我们的数据共同证明，唾液电动汽车带有信息蛋白，可通过无创方式用于检测肺癌。

更新日期：2018-02-14

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