High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.

高通量基因组测序已揭示了许多潜在的次生代谢产物生物合成途径，这些途径仍然是秘密的。通过异源表达的途径重建与基因工程相结合，使得能够发现新化合物，阐明生物合成途径以及优化产品产量。除大肠杆菌和酵母外，真菌，尤其是曲霉属菌，是众所周知的有效异源宿主。这篇综述总结了曲霉中微生物次生代谢产物生物合成异源表达的最新进展。spp。我们还讨论了微生物次生代谢产物生物合成重建背后的异源宿主选择和DNA组装方面的技术挑战和成功。