Title Quartz-Crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalization,Electroanalysis

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Title Quartz-Crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalization
Electroanalysis ( IF 2.7 ) Pub Date : 2018-02-05 , DOI: 10.1002/elan.201700789
Kutay Icoz ₁ , Mehmet Cagri Soylu ₂ , Zeynep Canikara ₂ , Ekrem Unal ₃

Affiliation

We have investigated different surface functionalization methods to immobilize CD19 antibody on gold surface to capture B lymphoblast cells associated with the acute lymphoblastic leukemia disease. Quartz Crystal Microbalance measurements were performed to analyze the binding kinetics of each layer and determine the optimum method, which results in higher cell capture rates. The random orientation of antibody and oriented antibody through protein G was investigated and protein G presence resulted in 15,2 Hz frequency shift for 10 cells/mL. The 3-mercaptopropyltrimethoxysilane (MPS) and 11-Mercaptoundecanoic acid (MUA) coatings of gold surface together with 4-(N-Maleimidomethyl) cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt (Sulfo-SMCC) and N-Ethyl-N’-(3dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysulfosuccinimide (NHS) linker layers were tested on QCM for protein G and antibody binding. The results indicate that MUA, EDC/NHS, protein G, antibody CD19 is the optimum surface modification among the tested combinations. By using the optimum surface functionalization method, minimum 10 cell per mL was measured as 1.9 Hz frequency shift.

中文翻译：

标题金表面 CD19 抗体固定和捕获 B 淋巴细胞的石英晶体微量平衡测量：表面功能化的影响

我们研究了不同的表面功能化方法，将 CD19 抗体固定在金表面上，以捕获与急性淋巴细胞白血病疾病相关的 B 淋巴细胞。进行石英晶体微量天平测量以分析每一层的结合动力学并确定最佳方法，从而提高细胞捕获率。研究了抗体和定向抗体通过蛋白 G 的随机取向，并且蛋白 G 的存在导致 10 个细胞/mL 的 15.2 Hz 频移。金表面的 3-巯基丙基三甲氧基硅烷 (MPS) 和 11-巯基十一烷酸 (MUA) 涂层以及 4-(N-马来酰亚胺甲基) 环己烷-1-羧酸 3-磺基-N-羟基琥珀酰亚胺酯钠盐 (Sulfo-SMCC) 和在 QCM 上测试了 N-乙基-N'-（3 二甲基氨基丙基）碳二亚胺盐酸盐 (EDC)/N-羟基磺基琥珀酰亚胺 (NHS) 接头层的蛋白 G 和抗体结合。结果表明 MUA、EDC/NHS、蛋白 G、抗体 CD19 是测试组合中最佳的表面修饰。通过使用最佳表面功能化方法，每毫升至少 10 个细胞被测量为 1.9 Hz 频移。抗体 CD19 是测试组合中最佳的表面修饰。通过使用最佳表面功能化方法，每毫升至少 10 个细胞被测量为 1.9 Hz 频移。抗体 CD19 是测试组合中最佳的表面修饰。通过使用最佳表面功能化方法，每毫升至少 10 个细胞被测量为 1.9 Hz 频移。

更新日期：2018-02-05

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