Monodispersed core-shell silica spheres with fibrous shell structure and tunable pore size were prepared by using a one-pot oil-water biphase method. The pore size could be tuned from 7 nm to 37 nm by using organic solvents with different polarities as oil phase. The spheres synthesized by using benzene as organic solvent had the maximum pore size of 37 nm and possessed a surface area of 61 m² g⁻¹. The obtained wide pore core-shell silica spheres were applied for rapidly separating small molecules, peptides, small proteins, and large proteins with molecular weight up to 200 kDa. Since the pore size of the core-shell silica spheres was sufficiently large for the free access of all the solutes, sharp and symmetric peaks were obtained. The separation performance was as high as 264,531 plates m⁻¹ for fluorene. The great efficient separation demonstrates that the wide pore core-shell silica spheres have a great potential for rapid analysis of both small and large solutes with high performance liquid chromatography.

采用一锅油水双相法制备了具有纤维壳结构和可调孔径的单分散核-壳二氧化硅球。可以使用极性不同的有机溶剂作为油相，将孔径从7 nm调整到37 nm。以苯为有机溶剂合成的球体最大孔径为37 nm，表面积为61 m ²  g ^-1。将获得的宽孔核-壳二氧化硅球用于快速分离分子量高达200 kDa的小分子，肽，小蛋白和大蛋白。由于核-壳二氧化硅球的孔径足够大，可以自由进入所有溶质，因此获得了尖锐且对称的峰。对于芴，分离性能高达264,531板m ^-1。高效的分离表明宽孔核-壳二氧化硅球具有使用高效液相色谱法快速分析大小溶质的巨大潜力。