The direct inactivation effects of an atmospheric pressure direct current (DC) air plasma against planktonic Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) in aqueous solution are investigated in vitro. Upon plasma treatment, extensively analyses on cell culturability, metabolic capacity, membrane integrity, surface morphology, cellular proteins, nucleic acids and intracellular reactive oxygen species (ROS) for both bacterial species were carried out and significant antimicrobial effects observed. Compared with the cellular culturability, a sub-lethal viable but non-culturable (VBNC) state was induced while more S. aureus entered this state than E. coli. Damaged bacterial outer structures were observed and the total concentrations of cellular protein and nucleic acid decreased for both bacteria after plasma treatment. The plasma-induced aqueous reactive species (RS) and intracellular ROS might produce detrimental effects to the bacteria, while S. aureus was less susceptible to the discharge after a 20-min exposure compared to E. coli.

一个大气压的直流（DC）空气等离子体对浮游的直接灭活效果的金黄色葡萄球菌（小号。金黄色葡萄球菌）和大肠杆菌（ê。大肠杆菌在水溶液中）在体外进行了研究。在血浆处理后，对两种细菌种类的细胞可培养性，代谢能力，膜完整性，表面形态，细胞蛋白，核酸和细胞内活性氧种类（ROS）进行了广泛分析，并观察到了显着的抗菌作用。与细胞培养性相比，亚致死活的非可培养（VBNC）状态下，同时更引起小号。金黄色比E进入此状态。大肠杆菌。观察到血浆处理后，两种细菌的​​细菌外部结构均受损，细胞蛋白和核酸的总浓度降低。血浆诱导的水反应性物种（RS）和细胞内ROS可能对细菌产生有害影响，而S则为细菌。与E相比，金黄色葡萄球在暴露20分钟后对放电的敏感性较小。大肠杆菌。