Hyperoxia is often used in the treatment of neonates. However, protracted use of hyperoxia leads to significant morbidity. The purpose of this study was to evaluate the effects of vitamin B-6 supplementation on oxidative stress and inflammatory responses in neonatal rats undergoing hyperoxia therapy. The study consisted of 2 parts: a survival study and a vitamin B-6 efficacy study for 16 days. Neonatal rats were randomly divided into either the control group, B-6 group (subcutaneously injected with 90 mg/kg/d of pyridoxal 5'-phosphate [PLP]), O2 group (treated with 85% oxygen), or O2 + B-6 group (simultaneously treated with 85% oxygen and 90 mg/kg/d PLP). After the survival study was done, the vitamin B-6 efficacy study was performed with duplicate neonatal rats sacrificed on the 3rd, 6th, 9th, and 16th day. Serum inflammatory cytokines, tissue pathology, and malondialdehyde (MDA) levels were measured. In the survival study, the survival rate of neonatal rats in the control, B-6, O2, and O2 + B-6 group on the 16th day were 100%, 100%, 25%, and 62.50%, respectively. The efficacy study showed lung polymorphonuclear granulocyte (PMN) and macrophage infiltration, increased liver hemopoiesis, and higher MDA levels in liver homogenates at days 3 through 16 in the O2 group. Vitamin B-6 supplementation considerably increased serum inflammatory cytokines in either the 6th or 9th day and decreased liver MDA level before the 6th day. These results indicate that neonatal rats receiving hyperoxia treatment suffered divergent serum inflammatory responses and were in increased liver oxidative stress. Vitamin B-6 supplementation seemed to improve survival rates, change systemic inflammatory response, and decrease liver oxidative stress while neonatal rats were under hyperoxia treatment.

中文翻译：

---

补充维生素B-6对高氧治疗新生大鼠氧化应激和炎症反应的影响

高氧常用于新生儿的治疗。然而，长期使用高氧会导致显着的发病率。本研究的目的是评估补充维生素 B-6 对接受高氧治疗的新生大鼠氧化应激和炎症反应的影响。该研究由两部分组成：生存研究和为期 16 天的维生素 B-6 功效研究。新生大鼠随机分为对照组、B-6 组（皮下注射 90 mg/kg/d 的 5'-磷酸吡哆醛 [PLP]）、O2 组（用 85% 氧气处理）或 O2 + B -6 组（同时用 85% 氧气和 90 mg/kg/d PLP 处理）。完成生存研究后，在第 3、6、9 和 16 天处死双份新生大鼠，进行维生素 B-6 功效研究。血清炎性细胞因子，组织病理学和丙二醛（MDA）水平进行了测量。在生存研究中，对照组、B-6、O2、O2+B-6组新生大鼠第16天的生存率分别为100%、100%、25%和62.50%。疗效研究显示，O2 组在第 3 天至第 16 天时，肺多形核粒细胞 (PMN) 和巨噬细胞浸润、肝脏造血增加和肝脏匀浆中的 MDA 水平升高。维生素 B-6 补充剂在第 6 天或第 9 天显着增加血清炎性细胞因子，并在第 6 天之前降低肝脏 MDA 水平。这些结果表明，接受高氧治疗的新生大鼠出现不同的血清炎症反应，并且肝脏氧化应激增加。补充维生素 B-6 似乎可以提高存活率，