The reversed-phase chromatographic behaviour of six tricyclic antidepressants (amitryptiline, clomipramine, doxepin, imipramine, nortryptiline and maprotiline) was examined in this work with acetonitrile-water mobile phases, in the absence and presence of the ionic liquids 1-hexyl-3-methylimidazolium chloride and 1-hexyl-3-methylimidazolium tetrafluoroborate, which have interesting features for the separation of basic compounds, in terms of peak shape combined with reduced retention. Tricyclic antidepressants are low polarity drugs that strongly associate to the alkyl chains of conventional stationary phases. They are also positively charged in the usual working pH range (2–8) in reversed-phase liquid chromatography, due to their strong basic character. In consequence, they may interact with the residual ionised silanols present in conventional silica-based stationary phases, which is translated in stronger retention, and tailed and broad peaks. A simple chromatographic procedure for the control of tricyclic antidepressants in pharmaceutical formulations was developed using a C8 column and a mobile phase containing 30% acetonitrile/10 mM 1-hexyl-3-methylimidazolium chloride at pH 3, with UV detection. Intra- and inter-day precisions were usually below +1.0%, and intra- and inter-day bias (trueness) ranged between ‒2.1% and +2.4%, and between ‒3.0% and +2.3%, respectively. Sample preparation was simple and only required solubilisation and filtration previous to injection.

在存在和不存在离子液体1-己基-3-的情况下，使用乙腈-水流动相检查了六种三环抗抑郁药（阿米替林，氯米帕明，多塞平，丙咪嗪，去甲替林和马普替林）的反相色谱行为。氯化甲基咪唑鎓盐和1-己基-3-甲基咪唑鎓四氟硼酸盐具有分离碱性化合物的有趣特征，其峰形与保留时间降低有关。三环抗抑郁药是与传统固定相的烷基链紧密缔合的低极性药物。由于它们的强碱性，在反相液相色谱中，它们在通常的工作pH范围（2–8）中也带正电。结果，它们可能与常规的基于硅胶的固定相中存在的残留离子化硅烷醇发生相互作用，从而转化为更强的保留力，并出现峰尾和宽峰。开发了一种简单的色谱方法，用于控制药物制剂中的三环抗抑郁药，方法是使用C8色谱柱和流动相，其中含有30％乙腈/ 10 mM 1-己基-3-甲基咪唑鎓氯化物，pH为3，并进行了UV检测。日内和日间精度通常低于+ 1.0％，日内和日间偏差（真实性）分别在‒2.1％和+ 2.4％之间以及‒3.0％和+ 2.3％之间。样品制备简单，只需在进样前溶解和过滤即可。开发了一种简单的色谱方法，用于控制药物制剂中的三环抗抑郁药，方法是使用C8色谱柱和流动相，其中含有30％乙腈/ 10 mM 1-己基-3-甲基咪唑鎓氯化物，pH为3，并进行了UV检测。日内和日间精度通常低于+ 1.0％，日内和日间偏差（真实性）分别在‒2.1％和+ 2.4％之间以及‒3.0％和+ 2.3％之间。样品制备很简单，在进样前只需要溶解和过滤即可。开发了一种简单的色谱方法，用于控制药物制剂中的三环抗抑郁药，方法是使用C8色谱柱和流动相，其中含有30％乙腈/ 10 mM 1-己基-3-甲基咪唑鎓氯化物，pH为3，并进行了UV检测。日内和日间精度通常低于+ 1.0％，日内和日间偏差（真实性）分别在‒2.1％和+ 2.4％之间以及‒3.0％和+ 2.3％之间。样品制备简单，只需在进样前溶解和过滤即可。日内和日间偏差（真实性）分别在‒2.1％和+ 2.4％之间以及‒3.0％和+ 2.3％之间。样品制备简单，只需在进样前溶解和过滤即可。日内和日间偏差（真实性）分别在‒2.1％和+ 2.4％之间以及‒3.0％和+ 2.3％之间。样品制备简单，只需在进样前溶解和过滤即可。