The baculovirus expression vector system (BEVS) is a popular manufacturing platform for the production of recombinant proteins, antiviral vaccines, gene therapy vectors, and biopesticides. Besides its successful applications in the industrial sector, the system has also played a significant role within the academic community given its extensive use in the production of hard-to-express eukaryotic multiprotein complexes for structural characterization for example. However, as other expression platforms, BEVS has to be continually improved to overcome its limitation and adapt to the constant demand for manufacturing processes that provide recombinant products with improved quality at higher yields and lower production cost.

RNA interference, or RNAi, is a relatively recent technology that has revolutionized how scientist study gene function. Originally introduced as a tool to study biological and disease-related processes it has recently been applied to improve the yield and quality of recombinant proteins produced in several expression systems. In this review, we provide a comprehensive summary of the impact that RNAi-mediated silencing of cellular or viral genes in the BEVS has on the production of recombinant products. We also propose a critical analysis of several aspects of the methodologies described in the literature for the use of RNAi technology in the BEVS with the intent to provide the reader with eventually useful guidance for designing experiments.

杆状病毒表达载体系统（BEVS）是生产重组蛋白，抗病毒疫苗，基因治疗载体和生物农药的流行制造平台。除了在工业领域的成功应用外，该系统还广泛用于生产难以表达的真核多蛋白复合物（例如，用于结构表征），在学术界中也发挥了重要作用。但是，作为其他表达平台，BEVS必须不断改进以克服其局限性，并适应对生产工艺的不断需求，以更高的产量和更低的生产成本提供具有更高质量的重组产品。

RNA干扰（RNAi）是一种相对较新的技术，它彻底改变了科学家研究基因功能的方式。最初是作为研究生物学和疾病相关过程的工具引入的，最近已被用于提高在几种表达系统中产生的重组蛋白的产量和质量。在这篇综述中，我们提供了BEVS中RNAi介导的细胞或病毒基因沉默对重组产物生产的影响的全面总结。我们还建议对在BEVS中使用RNAi技术的文献中描述的方法的几个方面进行批判性分析，目的是为读者提供设计实验的最终有用指导。