In recent years, circular RNAs (circRNAs) have been shown to have critical regulatory roles in cancer biology. However, the contributions of circRNAs to hepatocellular carcinoma (HCC) remain largely unknown. cSMARCA5 (a circRNA derived from exons 15 and 16 of the gene, hsa_circ_0001445) was identified by RNA-sequencing and validated by quantitative reverse transcription PCR. The role of cSMARCA5 in HCC progression was assessed both and . circRNAs precipitation, luciferase reporter assay, biotin-coupled microRNA capture and fluorescence hybridization were conducted to evaluate the interaction between cSMARCA5 and miR-17-3p/miR-181b-5p. The expression of cSMARCA5 was lower in HCC tissues, because of the regulation of DExH-Box Helicase 9, an abundant nuclear RNA helicase. The downregulation of cSMARCA5 in HCC was significantly correlated with aggressive characteristics and served as an independent risk factor for overall survival and recurrence-free survival in patients with HCC after hepatectomy. Our and data indicated that cSMARCA5 inhibits the proliferation and migration of HCC cells. Mechanistically, we found that cSMARCA5 could promote the expression of TIMP3, a well-known tumor suppressor, by sponging miR-17-3p and miR-181b-5p. These results reveal an important role of cSMARCA5 in the growth and metastasis of HCC and provide a fresh perspective on circRNAs in HCC progression. Herein, we studied the role of cSMARCA5, a circular RNA, in hepatocellular carcinoma. Our and data showed that cSMARCA5 inhibits the growth and migration of hepatocellular carcinoma cells, making it a potential therapeutic target.

中文翻译：

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环状RNA cSMARCA5抑制肝细胞癌的生长和转移

近年来，环状RNA（circRNA）已被证明在癌症生物学中具有关键的调节作用。然而，circRNA 对肝细胞癌 (HCC) 的贡献仍然很大程度上未知。 cSMARCA5（源自基因外显子 15 和 16 的 circRNA，hsa_circ_0001445）通过 RNA 测序进行鉴定，并通过定量逆转录 PCR 进行验证。评估了 cSMARCA5 在 HCC 进展中的作用。进行circRNA沉淀、荧光素酶报告基因测定、生物素偶联的microRNA捕获和荧光杂交来评估cSMARCA5和miR-17-3p/miR-181b-5p之间的相互作用。由于DExH-Box解旋酶9（一种丰富的核RNA解旋酶）的调节，cSMARCA5在HCC组织中的表达较低。 HCC 中 cSMARCA5 的下调与侵袭性特征显着相关，并且是肝切除术后 HCC 患者总生存期和无复发生存期的独立危险因素。我们的数据表明cSMARCA5抑制HCC细胞的增殖和迁移。从机制上讲，我们发现cSMARCA5可以通过海绵miR-17-3p和miR-181b-5p来促进众所周知的肿瘤抑制因子TIMP3的表达。这些结果揭示了 cSMARCA5 在 HCC 生长和转移中的重要作用，并为 circRNA 在 HCC 进展中提供了新的视角。在此，我们研究了环状 RNA cSMARCA5 在肝细胞癌中的作用。我们的数据显示，cSMARCA5 抑制肝细胞癌细胞的生长和迁移，使其成为潜在的治疗靶点。