Single‐Molecule Kinetics of an Enzyme in the Presence of Multiple Substrates,ChemBioChem

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Single‐Molecule Kinetics of an Enzyme in the Presence of Multiple Substrates
ChemBioChem ( IF 2.6 ) Pub Date : 2018-03-23 , DOI: 10.1002/cbic.201700695
Divya Singh ₁ , Srabanti Chaudhury ₁

Affiliation

Determining the sequence of binding: Three different mechanisms of bisubstrate binding to a single enzyme during catalysis are presented. The theoretical calculations of the mean turnover time and randomness parameter as a function of the substrate concentration can distinguish between different types of bisubstrate binding mechanisms.