Giant unilamellar vesicles (GUVs) have become one of extensively studied biological bilayer models especially when investigating topological and mechanical properties of cell membranes. They are also used to visualize membrane-related phenomena. However, the method of preparation and the effects of parameters of preparation on the vesicular structure are extensively varied. Therefore, it is important to understand how the process of formation of GUVs influences the outcome population, as it can influence the outcome of the experiment that is planned. Therefore, in this study, we investigated the effects of protocol parameters of electroformation on properties of homogeneous population of POPC GUVs. The parameters investigated in this study are duration of electroformation, usage of electrodes and frequency of applied AC field and its voltage. The properties investigated, which can be used to describe GUV populations are average diameter of vesicle, the amount of lipid molecules in population, and structure of vesicles. According to our results, prolonged time (greater than 4 h) does not influence outcome; however, parameters of applied electrical field (voltage and frequency) did significantly influence the properties of obtained POPC GUV populations.

巨大的单层囊泡（GUV）已成为广泛研究的生物双层模型之一，尤其是在研究细胞膜的拓扑和机械特性时。它们还用于可视化膜相关现象。然而，制备方法和制备参数对囊泡结构的影响广泛地变化。因此，重要的是要了解GUV的形成过程如何影响结果总体，因为它可以影响计划中的实验的结果。因此，在这项研究中，我们调查了电形成的协议参数对POPC GUV的同质种群的性质的影响。在这项研究中研究的参数是电形成的持续时间，电极的使用以及所施加的交流电场的频率及其电压。可以用来描述GUV种群的研究性质是囊泡的平均直径，种群中脂质分子的数量和囊泡的结构。根据我们的结果，延长时间（大于4小时）不会影响结果；但是，施加的电场参数（电压和频率）确实会显着影响获得的POPC GUV种群的性质。