Dental Materials ( IF 4.6 ) Pub Date : 2018-02-01 , DOI: 10.1016/j.dental.2018.01.006 Kunneng Liang , Shimeng Xiao , Hongling Liu , Wenyuan Shi , Jianshu Li , Yuan Gao , Libang He , Xuedong Zhou , Jiyao Li
Objective
Eight repetitive nucleotide sequences of aspartate–serine–serine (8DSS) derived from dentin phosphoprotein (DPP) has been proved to be a good remineralization agency. In this study, 8DSS peptide was employed to induce dentinal tubule occlusion.
Methods
Dentin samples were acid-etched, and then the samples were coated with 8DSS solution. The binding capacity of 8DSS to acid-etched dentin was tested by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Subsequently, the 8DSS-treated dentin samples were immersed in artificial saliva for 1, 2 and 4 weeks. After 4 weeks, the remineralized dentin was treated with 6 wt% citric acid (pH 1.5) solution for 1 min. Dentin permeability measurement and scanning electron microscopy (SEM) were carried out after different periods. Energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD) were used to identify the mineral phase of the regenerated minerals.
Results
The results showed that 8DSS had a good binding capacity to the acid-etched dentin, and significantly reduced the dentin permeability by inducing minerals deposited within the dentinal tubules. After 4 weeks, all the dentinal tubules were occluded by large bulk of regenerated minerals, which largely decreased the diameters of the tubules. The regenerated minerals deposited with a deep depth within the dentinal tubules, ensuring an effective occlusion even after an acid challenge. The results of XRD and EDS confirmed that the regenerated minerals were mainly hydroxyapatite (HA).
Significance
8DSS peptide induced strong dentinal tubule occlusion. 8DSS have a great potential to be used in the treatment of dentin hypersensitivity in the future.
中文翻译:
8DSS肽体外诱导有效的牙本质小管阻塞
客观的
来自牙本质磷蛋白(DPP)的八种天冬氨酸-丝氨酸-丝氨酸(8DSS)的重复核苷酸序列已被证明是很好的再矿化剂。在这项研究中,采用8DSS肽诱导牙本质小管阻塞。
方法
酸蚀牙本质样品,然后用8DSS溶液涂覆样品。通过衰减全反射傅里叶变换红外光谱(ATR-FTIR)测试了8DSS与酸蚀牙本质的结合能力。随后,将经过8DSS处理的牙本质样品浸入人工唾液中1、2和4周。4周后,将再矿化的牙本质用6重量%柠檬酸(pH 1.5)溶液处理1分钟。在不同的时期后进行牙本质渗透性测量和扫描电子显微镜(SEM)。能量色散光谱(EDS)和X射线衍射(XRD)用于鉴定再生矿物的矿物相。
结果
结果表明8DSS对酸蚀的牙本质具有良好的结合能力,并通过诱导沉积在牙本质小管内的矿物质显着降低了牙本质的渗透性。4周后,所有牙本质小管都被大量的再生矿物质阻塞,这大大减小了小管的直径。再生的矿物质在牙本质小管内深处沉积,即使在酸刺激后也能确保有效的阻塞。XRD和EDS的结果证实,再生矿物主要为羟基磷灰石(HA)。
意义
8DSS肽诱导强力的牙本质小管阻塞。8DSS将来有很大的潜力可用于治疗牙本质过敏症。