Purpose

To describe the study protocol and baseline characteristics of the African Descent and Glaucoma Evaluation Study (ADAGES) III.

Design

Cross-sectional, case-control study.

Participants

Three thousand two hundred sixty-six glaucoma patients and control participants without glaucoma of African or European descent were recruited from 5 study centers in different regions of the United States.

Methods

Individuals of African descent (AD) and European descent (ED) with primary open-angle glaucoma (POAG) and control participants completed a detailed demographic and medical history interview. Standardized height, weight, and blood pressure measurements were obtained. Saliva and blood samples to provide serum, plasma, DNA, and RNA were collected for standardized processing. Visual fields, stereoscopic disc photographs, and details of the ophthalmic examination were obtained and transferred to the University of California, San Diego, Data Coordinating Center for standardized processing and quality review.

Main Outcome Measures

Participant gender, age, race, body mass index, blood pressure, history of smoking and alcohol use in POAG patients and control participants were described. Ophthalmic measures included intraocular pressure, visual field mean deviation, central corneal thickness, glaucoma medication use, or past glaucoma surgery. Ocular conditions, including diabetic retinopathy, age-related macular degeneration, and past cataract surgery, were recorded.

Results

The 3266 ADAGES III study participants in this report include 2146 AD POAG patients, 695 ED POAG patients, 198 AD control participants, and 227 ED control participants. The AD POAG patients and control participants were significantly younger (both, 67.4 years) than ED POAG patients and control participants (73.4 and 70.2 years, respectively). After adjusting for age, AD POAG patients had different phenotypic characteristics compared with ED POAG patients, including higher intraocular pressure, worse visual acuity and visual field mean deviation, and thinner corneas (all P < 0.001). Family history of glaucoma did not differ between AD and ED POAG patients.

Conclusions

With its large sample size, extensive specimen collection, and deep phenotyping of AD and ED glaucoma patients and control participants from different regions in the United States, the ADAGES III genomics study will address gaps in our knowledge of the genetics of POAG in this high-risk population.

中文翻译：

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非洲人后裔和青光眼评估研究（ADAGES）III ：非裔美国人基因型对青光眼表型的贡献：研究设计和基线数据

目的

描述非洲人后裔和青光眼评估研究（ADAGES）III的研究方案和基线特征。

设计

横断面病例对照研究。

参加者

从美国不同地区的5个研究中心招募了362名青光眼患者和没有非洲或欧洲血统的青光眼的对照组。

方法

患有原发性开角型青光眼（POAG）的非洲人后裔（AD）和欧洲人后裔（ED）和对照组参与者完成了详细的人口统计学和病史采访。获得了标准化的身高，体重和血压测量值。收集唾液和血液样本以提供血清，血浆，DNA和RNA，以进行标准化处理。获得了视野，立体视盘照片和眼科检查的详细信息，并将其转移到加利福尼亚大学圣地亚哥分校的数据协调中心，以进行标准化处理和质量检查。

主要观察指标

描述了POAG患者和对照组参与者的参与者性别，年龄，种族，体重指数，血压，吸烟和饮酒史。眼科测量包括眼内压，视野平均偏差，中央角膜厚度，青光眼药物的使用或过去的青光眼手术。记录眼病，包括糖尿病性视网膜病，与年龄有关的黄斑变性和过去的白内障手术。

结果

该报告中的3266位ADAGES III研究参与者包括2146位AD POAG患者，695位ED POAG患者，198位AD对照参与者和227位ED对照参与者。AD POAG患者和对照组（分别为67.4岁）比ED POAG患者和对照组（分别为73.4岁和70.2岁）年轻。调整年龄后，AD POAG患者与ED POAG患者具有不同的表型特征，包括较高的眼内压，较差的视敏度和视野平均偏差以及较薄的角膜（所有P <0.001）。AD和ED POAG患者之间青光眼的家族史无差异。

结论

ADAGES III基因组学研究样本量大，标本收集广泛，并且对来自美国不同地区的AD和ED青光眼患者以及对照组参与者进行了深表型分析，因此ADAGES III基因组学研究将填补我们在这一高水平人群中对POAG遗传学知识的不足。危险人群。