3D cellular structures and co-cultures formed through the contactless magnetic manipulation of cells on adherent surfaces,Biomaterials Science

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3D cellular structures and co-cultures formed through the contactless magnetic manipulation of cells on adherent surfaces
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-02-01 00:00:00 , DOI: 10.1039/c7bm01050h
Abdel Rahman Abdel Fattah _{1,

2,

3,

4} , Sarah Mishriki _{2,

3,

4,

5} , Tobias Kammann _{6,

7,

8} , Rakesh P. Sahu _{1,

2,

3,

4} , Fei Geng _{1,

2,

3,

4} , Ishwar K. Puri _{1,

2,

3,

4,

5}

Affiliation

A magnet array is employed to manipulate diamagnetic cells that are contained in paramagnetic medium to demonstrate for the first time the contactless bioprinting of three-dimensional (3D) cellular structures and co-cultures of breast cancer MCF-7 and endothelial HUVEC at prescribed locations on tissue culture treated well plates. Sequential seeding of different cell lines and the spatial displacement of the magnet array creates co-cultured cellular structures within a well without using physically intrusive well inserts. Both monotypic and co-culture experiments produce morphologically rich 3D cell structures that are otherwise absent in regular monolayer cell cultures. The magnetic contactless bioprinting of cells provides further insight into cell behaviour, invasion strategies and transformations that are useful for potential applications in drug screening, 3D cell culture formation and tissue engineering.

中文翻译：

3D细胞结构和共培养物是通过粘附表面上细胞的非接触式磁操作形成的

使用磁体阵列来操纵顺磁性介质中包含的反磁性细胞，以首次展示乳腺癌MCF-7和内皮HUVEC的三维（3D）细胞结构以及共培养物的非接触式生物印记。组织培养处理好的孔板。不同细胞系的顺序播种和磁体阵列的空间位移可在不使用物理侵入性孔插入物的情况下在孔内创建共培养的细胞结构。单型和共培养实验均产生形态丰富的3D细胞结构，而常规单层细胞培养中则没有这种结构。细胞的磁性非接触式生物印记可进一步了解细胞行为，

更新日期：2018-02-01

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