A facile and sensitive detection of L-cysteine is essential for biosensors, environmental monitoring and medical diagnosis. In this study, a simple approach has been demonstrated to prepare Prussian blue (PB)/polypyrrole (PPy) hybrid nanofibers as efficient peroxidase-like mimics for the determination of L-cysteine. α-Fe₂O₃ nanofibers act as both templates and a source of Fe³⁺ to polymerize pyrrole in an acidic environment, resulting in the formation of PB/PPy hybrid nanofibers. The as-prepared PB/PPy hybrid nanofibers show a superior catalytic activity as peroxidase mimics over α-Fe₂O₃ nanofibers. Due to the high catalytic efficiency of the resultant PB/PPy hybrid nanofibers, we have developed a simple colorimetric approach for the rapid and sensitive determination of L-cysteine with a detection limit of about 16 nM over a wide linear range from 1 to 20 μM. In addition, the detection system displays a high selectivity toward L-cysteine over other amino acids, glucose, and common ions. This work provides a new strategy for the preparation of peroxidase-like nanomaterials with improved catalytic activity, which shows great promise for application in highly sensitive detection.

中文翻译：

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简便地制备普鲁士蓝/聚吡咯杂化纳米纤维，作为鲁棒的过氧化物酶模拟物，用于比色检测l-半胱氨酸

简便，灵敏地检测L-半胱氨酸对于生物传感器，环境监测和医学诊断至关重要。在这项研究中，已经证明了一种简单的方法可以制备普鲁士蓝（PB）/聚吡咯（PPy）杂化纳米纤维，作为测定L-半胱氨酸的有效过氧化物酶样模拟物。的α-Fe _2个ö ₃纳米纤维作为模板和Fe的源³⁺在酸性环境中进行聚合吡咯，导致PB /聚吡咯的纳米纤维混合的形成。所制备的PB /聚吡咯的纳米纤维混合显示出优良的催化活性，在过氧化物酶模拟物的α-Fe ₂ ö ₃纳米纤维。由于所得PB / PPy杂合纳米纤维的高催化效率，我们开发了一种简单的比色法，可快速灵敏地测定L-半胱氨酸，在1至20μM的宽线性范围内，检出限约为16 nM。 。另外，该检测系统对L-半胱氨酸显示出比其他氨基酸，葡萄糖和常见离子更高的选择性。这项工作为制备具有改善的催化活性的过氧化物酶样纳米材料提供了一种新的策略，这为在高灵敏度检测中的应用展示了广阔的前景。