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Sampling from Single Cells
Small Methods ( IF 10.7 ) Pub Date : 2018-01-26 , DOI: 10.1002/smtd.201700300 Paolo Actis 1
Small Methods ( IF 10.7 ) Pub Date : 2018-01-26 , DOI: 10.1002/smtd.201700300 Paolo Actis 1
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The cell is the fundamental unit of biology. Major methodological advances in engineering and molecular biology have enabled the 'omics analysis of individual cells and supported biologists in understanding the deepest difference between health and disease. These advancements were based on the assumption that a single cell needs to be lysed or fixed before any in‐depth analysis can be performed. Here, a picture is painted of the innovative methods used for extracting the contents of living cells without affecting their viability. These novel methods are now empowering the biological community to repeatedly interrogate a single cell over time, thus giving a dynamic representation of the cell's 'omics rather than a snapshot at a particular time point.
中文翻译：
从单细胞采样
细胞是生物学的基本单位。工程学和分子生物学的主要方法学进展已使单个细胞的组学分析成为可能，并支持生物学家了解健康与疾病之间的最深层差异。这些进展是基于这样的假设，即在执行任何深入分析之前，都需要裂解或固定单个细胞。在这里，描绘了用于提取活细胞内容物而又不影响其生存能力的创新方法。这些新颖的方法现在使生物群落能够随时间反复询问单个细胞，从而动态显示细胞的组学信息，而不是特定时间点的快照。
更新日期：2018-01-26
中文翻译：
从单细胞采样
细胞是生物学的基本单位。工程学和分子生物学的主要方法学进展已使单个细胞的组学分析成为可能，并支持生物学家了解健康与疾病之间的最深层差异。这些进展是基于这样的假设，即在执行任何深入分析之前，都需要裂解或固定单个细胞。在这里，描绘了用于提取活细胞内容物而又不影响其生存能力的创新方法。这些新颖的方法现在使生物群落能够随时间反复询问单个细胞，从而动态显示细胞的组学信息，而不是特定时间点的快照。
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