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Preparation of molecularly imprinted hydrogel layer SPR sensor chips with lectin-recognition sites via SI-ATRP
Polymer Journal ( IF 2.3 ) Pub Date : 2018-01-25 , DOI: 10.1038/s41428-017-0013-7
Rinyarat Naraprawatphong , Akifumi Kawamura , Takashi Miyata

AbstractMolecularly imprinted hydrogel layers with lectin-recognition sites were prepared on surface plasmon resonance (SPR) sensor chips via surface-initiated atom transfer radical polymerization (SI-ATRP) combined with molecular imprinting. The lectin-imprinted hydrogel layer sensor chips showed larger SPR signal change in response to a target lectin than nonimprinted hydrogel layer sensor chips. The larger SPR signal change was attributed to the strong affinity constant of the lectin-imprinted hydrogel layer for the target lectin. These results suggest that molecular recognition sites for the lectin were formed within the hydrogel layers by molecular imprinting. On the other hand, the SPR signal change of the lectin-imprinted hydrogel layer chip in the presence of other lectin was very small. Poly(2-methacryloxyethyl phosphorylcholine) as a main chain of the hydrogel layer inhibited nonspecific adsorption of other lectin. This paper describes that SI-ATRP with biomolecular imprinting is a useful method to design highly sensitive and selective SPR sensor chips with molecular recognition sites for a target lectin.Molecularly imprinted hydrogel layer SPR sensor chips with lectin-recognition sites, which were prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) combined with molecular imprinting, exhibited not only large SPR signal change in response to a target lectin but also inhibited nonspecific protein adsorption.

中文翻译:

通过SI-ATRP制备具有凝集素识别位点的分子印迹水凝胶层SPR传感器芯片

摘要 通过表面引发的原子转移自由基聚合(SI-ATRP)结合分子印迹,在表面等离子体共振(SPR)传感器芯片上制备了具有凝集素识别位点的分子印迹水凝胶层。与非印迹水凝胶层传感器芯片相比,凝集素印迹水凝胶层传感器芯片响应目标凝集素显示出更大的 SPR 信号变化。较大的 SPR 信号变化归因于凝集素印迹水凝胶层对目标凝集素的强亲和力常数。这些结果表明凝集素的分子识别位点是通过分子印迹在水凝胶层内形成的。另一方面,在存在其他凝集素的情况下,凝集素印迹的水凝胶层芯片的 SPR 信号变化非常小。聚(2-甲基丙烯酰氧乙基磷酸胆碱)作为水凝胶层的主链,抑制了其他凝集素的非特异性吸附。本文描述了具有生物分子印迹的 SI-ATRP 是设计具有目标凝集素分子识别位点的高灵敏度和选择性 SPR 传感器芯片的有用方法。启动原子转移自由基聚合(SI-ATRP)与分子印迹相结合,不仅表现出响应目标凝集素的大 SPR 信号变化,而且还抑制了非特异性蛋白质吸附。
更新日期:2018-01-25
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