Feasibility of Defect Tunable Bone Engineering Using Electroblown Bioactive Fibrous Scaffolds with Dental Stem Cells,ACS Biomaterials Science & Engineering

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Feasibility of Defect Tunable Bone Engineering Using Electroblown Bioactive Fibrous Scaffolds with Dental Stem Cells
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-01-25 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00810
Nandin Mandakhbayar _{1,

2} , Ahmed El-Fiqi _{1,

2,

3} , Khandmaa Dashnyam _{1,

2} , Hae-Won Kim _{1,

2,

4}

Affiliation

Healing and repair of damaged bones with various geometries are challenging issues in personalized regenerative medicine. Herein, we examine if the engineered electroblown scaffolds can be suitable to this purpose with the ability to shape and fill defects, populate stem cells, and stimulate the regeneration process of defected bone. The electroblowing method could generate bioactive nanocomposite scaffolds made of poly(caprolactone) and bioactive glass nanoparticles, of which the macrostructure is highly spaced and fibrous networked. The scaffolds were easily formable to different shapes with space filling ability, and were hydrophilic to soak water and blood rapidly. Multipotent stem cells from dental pulp effectively infiltrated the scaffold networks, anchored the fiber surface within few hours, proliferated actively over weeks, and were stimulated to differentiate into osteogenic cells. The cell/scaffold constructs, implanted to tooth-extracted, irregular-shaped alveolar bone defects, were shown to fit the defect and to stimulate early new bone formation. Taken together, the electroblown bioactive fibrous scaffolds and their constructs with multipotent dental stem cells may be useful as a potential 3D platform for future personalized bone tissue engineering.

中文翻译：

使用电吹生物活性纤维支架和牙科干细胞进行缺损可调骨工程的可行性

具有各种几何形状的受损骨骼的修复和修复在个性化再生医学中是具有挑战性的问题。本文中，我们检查了工程改造的电喷支架是否适合于此目的，该支架具有塑造和填充缺损，填充干细胞以及刺激缺损骨再生的能力。电吹方法可以产生由聚己内酯和生物活性玻璃纳米粒子制成的生物活性纳米复合材料支架，它们的宏观结构是高度间隔的并且是纤维状的。支架易于形成具有空间填充能力的不同形状，并且具有亲水性以迅速吸收水和血液。来自牙髓的多能干细胞有效地渗透到支架网络中，在数小时内锚定了纤维表面，并在数周内活跃增殖，并被刺激分化为成骨细胞。植入到牙齿提取的不规则形状的牙槽骨缺损中的细胞/支架构建体显示出可以适应缺损并刺激早期新骨形成。综上所述，电吹生物活性纤维支架及其具有多能性牙干细胞的构建体可用作未来个性化骨组织工程的潜在3D平台。

更新日期：2018-01-25

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