Target Confinement in Small Reaction Volumes Using Microfluidic Technologies: A Smart Approach for Single-Entity Detection and Analysis,ACS Sensors

当前位置： X-MOL 学术 › ACS Sens. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Target Confinement in Small Reaction Volumes Using Microfluidic Technologies: A Smart Approach for Single-Entity Detection and Analysis
ACS Sensors ( IF 8.2 ) Pub Date : 2018-02-03 00:00:00 , DOI: 10.1021/acssensors.7b00873
Karen Ven ₁ , Bram Vanspauwen ₁ , Elena Pérez-Ruiz ₁ , Karen Leirs ₁ , Deborah Decrop ₁ , Hans Gerstmans _{1,

2,

3} , Dragana Spasic ₁ , Jeroen Lammertyn ₁

Affiliation

Over the last decades, the study of cells, nucleic acid molecules, and proteins has evolved from ensemble measurements to so-called single-entity studies. The latter offers huge benefits, not only as biological research tools to examine heterogeneities among individual entities within a population, but also as biosensing tools for medical diagnostics, which can reach the ultimate sensitivity by detecting single targets. Whereas various techniques for single-entity detection have been reported, this review focuses on microfluidic systems that physically confine single targets in small reaction volumes. We categorize these techniques as droplet-, microchamber-, and nanostructure-based and provide an overview of their implementation for studying single cells, nucleic acids, and proteins. We furthermore reflect on the advantages and limitations of these techniques and highlight future opportunities in the field.

中文翻译：

使用微流控技术在小反应体积中进行目标限制：单实体检测和分析的智能方法

在过去的几十年中，对细胞，核酸分子和蛋白质的研究已从整体测量发展到所谓的单实体研究。后者不仅提供了巨大的优势，不仅可以作为研究人群中个体间异质性的生物学研究工具，而且还可以作为医学诊断的生物传感工具，通过检测单个目标可以达到最终的灵敏度。尽管已经报道了用于单实体检测的各种技术，但本综述着重于将单个靶标物理限制在小反应体积中的微流体系统。我们将这些技术分为基于液滴，微室和纳米结构的技术，并概述了它们用于研究单细胞，核酸和蛋白质的实现方法。

更新日期：2018-02-03

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11