Ampicillin is a one of effective antibiotics against Gram‐positive and Gram‐negative bacteria. This study aimed to label ampicillin‐loaded graphene oxide nanoflake (AMP‐GO) with ^99mTc and evaluate of its in vitro binding to Staphylococcus aureus and Escherichia coli. Firstly, ampicillin was loaded into graphene oxide nanoflake prepared. AMP‐GO was characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) techniques, and the amount of loaded ampicillin onto GO was determined by UV‐Vis absorption spectroscopy. AMP and AMP‐GO were labeled with ^99mTc using stannous chloride reducing agent. Labeling efficiency of ^99mTc‐AMP‐GO was found to be 97.66 ± 2.06%. ^99mTc‐AMP‐GO has higher binding efficiencies to both S. aureus and E. coli than ^99mTc‐AMP. ^99mTc‐AMP‐GO could be promising candidate as agent infection nuclear imaging. Furthermore, in vivo studies of ^99mTc‐AMP‐GO with infected rats are planned to be performed.

中文翻译：

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放射性标记的氨苄青霉素氧化石墨烯纳米薄片的制备及其对大肠杆菌和金黄色葡萄球菌的作用评估

氨苄西林是对抗革兰氏阳性和革兰氏阴性细菌的有效抗生素之一。这项研究旨在用^99m Tc标记负载氨苄青霉素的氧化石墨烯纳米片（AMP-GO），并评估其与金黄色葡萄球菌和大肠杆菌的体外结合。首先，将氨苄西林加载到制备的氧化石墨烯纳米片中。AMP‐GO通过傅立叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）技术进行了表征，而氨苄青霉素在GO上的负载量通过UV‐Vis吸收光谱法进行了测定。AMP和AMP‐GO使用氯化亚锡还原剂标记为^99m Tc。发现^99m Tc-AMP-GO的标记效率为97.66±2.06％。^99m Tc-AMP-GO对金黄色葡萄球菌和大肠杆菌的结合效率均高于^99m Tc-AMP。^99m Tc-AMP-GO有望成为病原体感染核成像的候选者。此外，计划对受感染大鼠进行^99m Tc-AMP-GO的体内研究。