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Silicon nanohybrid-based SERS chips armed with an internal standard for broad-range, sensitive and reproducible simultaneous quantification of lead(ii) and mercury(ii) in real systems†
Nanoscale ( IF 5.8 ) Pub Date : 2018-01-24 00:00:00 , DOI: 10.1039/c7nr07935d
Yu Shi 1, 2, 3, 4, 5 , Na Chen 1, 2, 3, 4, 5 , Yuanyuan Su 1, 2, 3, 4, 5 , Houyu Wang 1, 2, 3, 4, 5 , Yao He 1, 2, 3, 4, 5
Affiliation  

Lead ions (Pb2+) and mercury ions (Hg2+), the two commonly coexisting heavy metal ions, pose severe risks to environment and human health. To date, no surface-enhanced Raman scattering (SERS) sensor has been reported for the simultaneous quantification of Pb2+ and Hg2+ in real systems. Herein, the first demonstration of SERS chips for simultaneous quantification of Pb2+ and Hg2+ in real systems is presented based on the combination of reproducible silicon nanohybrid substrates and a corrective internal standard (IS) sensing strategy. This chip was made of a silver nanoparticle-decorated silicon wafer via modification of the IS, i.e. 4-aminothiophenol, molecules. The as-prepared chip was further functionalized with Pb2+- and Hg2+- specific DNA strands capable of simultaneously detecting Pb2+ and Hg2+. Quantitatively, upon correction by the IS Raman signals, the broad dynamic ranges from 100 pM to 10 μM for Pb2+ and from 1 nM to 10 μM for Hg2+ were achieved, with the detection limit down to 19.8 ppt for Pb2+ and 168 ppt for Hg2+. For real applications, we further demonstrated that Pb2+ and Hg2+ spiked into industrial wastewater could be readily distinguished via the presented chip, and the relative standard deviation (RSD) value was less than ∼15%. More significantly, the resulting SERS chip can be well coupled with a hand-held Raman instrument and can then be used for the qualitative analysis of both Pb2+ and Hg2+ in real systems in a portable manner. Our results suggest that this high-quality SERS chip is a powerful tool for on-site detection of various heavy metal ions in real samples in the field of food safety and environment protection.

中文翻译:

基于硅纳米杂化的SERS芯片,其内部标准可对真实系统中的铅(ii)和汞(ii)进行宽范围,灵敏且可重现的同时定量

铅离子(Pb 2+)和汞离子(Hg 2+)是两种常见的重金属离子,对环境和人类健康构成严重威胁。迄今为止,还没有报道用于在实际系统中同时定量Pb 2+和Hg 2+的表面增强拉曼散射(SERS)传感器。本文中,基于可重现的硅纳米杂化底物和校正内标(IS)传感策略的结合,展示了用于在真实系统中同时定量Pb 2+和Hg 2+的SERS芯片的首次演示。该芯片是通过对IS进行修改而由装饰有纳米银颗粒的硅晶片制成的,4-氨基硫酚,分子。所制备的芯片与铅被进一步官能2+ -和Hg 2+ -特定的DNA链能够同时检测的Pb 2+和Hg 2+。定量地,当校正由IS拉曼信号中,宽动态范围为100pM至10μM对Pb 2+和从1nM至10μM的对Hg 2+被实现,以检测极限降低到19.8个百分点对Pb 2+2+为168 ppt 。对于实际应用中,我们进一步证实了铅2+和Hg 2+掺入工业废水可以容易地区别通过芯片,相对标准偏差(RSD)值小于15%。更重要的是,所得的SERS芯片可与手持式拉曼仪器良好耦合,然后可用于便携式系统中的Pb 2+和Hg 2+的定性分析。我们的结果表明,这种高质量的SERS芯片是在食品安全和环境保护领域中现场检测实际样品中各种重金属离子的有力工具。
更新日期:2018-01-24
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