Biosynthesis and Characterization of Polyhydroxyalkanoates with Controlled Composition and Microstructure,Biomacromolecules

当前位置： X-MOL 学术 › Biomacromolecules › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Biosynthesis and Characterization of Polyhydroxyalkanoates with Controlled Composition and Microstructure
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-01-23 00:00:00 , DOI: 10.1021/acs.biomac.7b01788
Anna Ferre-Guell ₁ , James Winterburn ₁

Affiliation

Volatile fatty acids (VFA) C2:0 to C6:0 were used as the sole carbon source for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production with controllable composition and microstructure in Haloferax mediterranei. Feeding carbon-even VFA gave >90 mol % poly(3-hydroxybutyrate) (3HB) PHBV, while carbon-odd VFA generated >87 mol % poly(3-hydroxyvalerate) (3HV) PHBV. Bespoke random, block, and blend copolymers with 0–100 mol % 3HV were synthesized using C4:0/C5:0 mixtures. The copolymer 3HV fraction is proportional to the %C5:0 in the feed mixture, allowing control over copolymer composition. Microstructure depends on the substrate addition order: cofeeding generated random copolymers, while sequential feeding created block and blend copolymers. On average, the PHBV had an ultrahigh molecular weight of 3 × 10⁶ g/mol. 3HV rich copolymers showed lower melting temperatures, enhanced elasticity, and ductility. H. mediterranei is ideal for large-scale production of PHBV due to its inherent bioprocessing advantages, while control over the composition and microstructure of PHBV will facilitate the production of biopolymers capable of meeting industrial criteria for specific applications.

中文翻译：

具有受控组成和微观结构的聚羟基链烷酸酯的生物合成和表征

挥发性脂肪酸（VFA）C2：0〜C6：0被用作聚（3-羟基丁酸酯唯一碳源共-3-羟基戊酸酯）（PHBV）的生产在可控组成和微观结构沃地中海。进料碳均匀的VFA得到> 90 mol％的聚（3-羟基丁酸酯）（3HB）PHBV，而碳奇数的VFA产生了> 87 mol％的聚（3-羟基戊酸酯）（3HV）PHBV。使用C4：0 / C5：0混合物合成了定制的具有0-100 mol％3HV的无规，嵌段和共混共聚物。共聚物的3HV分数与进料混合物中的％C5：0成比例，从而可以控制共聚物的组成。微观结构取决于基材的添加顺序：共进料生成无规共聚物，而顺序进料生成嵌段和共混共聚物。平均而言，PHBV具有3×10 ⁶ g / mol的超高分子量。富含3HV的共聚物显示出较低的熔融温度，增强的弹性和延展性。地中海 由于PHBV固有的生物加工优势，是大规模生产PHBV的理想选择，同时控制PHBV的组成和微观结构将有助于生产能够满足特定应用工业标准的生物聚合物。

更新日期：2018-01-23

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11