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Metabolic Fate of 13C-Labeled Polydextrose and Impact on the Gut Microbiome: A Triple-Phase Study in a Colon Simulator
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-02-20 00:00:00 , DOI: 10.1021/acs.jproteome.7b00683 Santosh Lamichhane 1, 2 , Christian C. Yde 1, 3 , Henrik Max Jensen 3 , Wesley Morovic 4 , Ashley A. Hibberd 4 , Arthur C. Ouwehand 5 , Markku T. Saarinen 5 , Sofia D. Forssten 5 , Lars Wiebe 6 , Jørn Marcussen 3 , Kresten Bertelsen 3, 7 , Sebastian Meier 8 , Jette F. Young 1 , Hanne Christine Bertram 1
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-02-20 00:00:00 , DOI: 10.1021/acs.jproteome.7b00683 Santosh Lamichhane 1, 2 , Christian C. Yde 1, 3 , Henrik Max Jensen 3 , Wesley Morovic 4 , Ashley A. Hibberd 4 , Arthur C. Ouwehand 5 , Markku T. Saarinen 5 , Sofia D. Forssten 5 , Lars Wiebe 6 , Jørn Marcussen 3 , Kresten Bertelsen 3, 7 , Sebastian Meier 8 , Jette F. Young 1 , Hanne Christine Bertram 1
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The present study introduces a novel triple-phase (liquids, solids, and gases) approach, which employed uniformly labeled [U–13C] polydextrose (PDX) for the selective profiling of metabolites generated from dietary fiber fermentation in an in vitro colon simulator using human fecal inocula. Employing 13C NMR spectroscopy, [U–13C] PDX metabolism was observed from colonic digest samples. The major 13C-labeled metabolites generated were acetate, butyrate, propionate, and valerate. In addition to these short-chain fatty acids (SCFAs), 13C-labeled lactate, formate, succinate, and ethanol were detected in the colon simulator samples. Metabolite formation and PDX substrate degradation were examined comprehensively over time (24 and 48 h). Correlation analysis between 13C NMR spectra and gas production confirmed the anaerobic fermentation of PDX to SCFAs. In addition, 16S rRNA gene analysis showed that the level of Erysipelotrichaceae was influenced by PDX supplementation and Erysipelotrichaceae level was statistically correlated with SCFA formation. Overall, our study demonstrates a novel approach to link substrate fermentation and microbial function directly in a simulated colonic environment.
中文翻译：
13 C标签聚右旋糖的代谢命运及其对肠道微生物组的影响：在结肠模拟器中进行的三阶段研究
本研究介绍了一种新颖的三相（液体，固体和气体）方法，该方法采用统一标记的[U– 13 C]聚葡萄糖（PDX）在体外结肠模拟器中对膳食纤维发酵产生的代谢产物进行选择性分析用人类粪便接种。使用13 C NMR光谱，从结肠消化液样品中观察到[U– 13 C] PDX代谢。产生的主要13种C标记代谢物是乙酸盐，丁酸盐，丙酸盐和戊酸盐。除了这些短链脂肪酸（SCFA），13在结肠模拟器样品中检测到了C标记的乳酸盐，甲酸盐，琥珀酸盐和乙醇。随着时间的推移（24和48小时）全面检查了代谢物的形成和PDX底物的降解。13 C NMR光谱与产气之间的相关分析证实了PDX到SCFAs的厌氧发酵。另外，16S rRNA基因分析表明，水平丹毒丝通过PDX补充和影响丹毒丝水平SCFA形成有统计学相关。总的来说，我们的研究表明了一种在模拟的结肠环境中直接链接底物发酵和微生物功能的新方法。
更新日期：2018-02-21
中文翻译：
13 C标签聚右旋糖的代谢命运及其对肠道微生物组的影响：在结肠模拟器中进行的三阶段研究
本研究介绍了一种新颖的三相（液体，固体和气体）方法，该方法采用统一标记的[U– 13 C]聚葡萄糖（PDX）在体外结肠模拟器中对膳食纤维发酵产生的代谢产物进行选择性分析用人类粪便接种。使用13 C NMR光谱，从结肠消化液样品中观察到[U– 13 C] PDX代谢。产生的主要13种C标记代谢物是乙酸盐，丁酸盐，丙酸盐和戊酸盐。除了这些短链脂肪酸（SCFA），13在结肠模拟器样品中检测到了C标记的乳酸盐，甲酸盐，琥珀酸盐和乙醇。随着时间的推移（24和48小时）全面检查了代谢物的形成和PDX底物的降解。13 C NMR光谱与产气之间的相关分析证实了PDX到SCFAs的厌氧发酵。另外，16S rRNA基因分析表明，水平丹毒丝通过PDX补充和影响丹毒丝水平SCFA形成有统计学相关。总的来说，我们的研究表明了一种在模拟的结肠环境中直接链接底物发酵和微生物功能的新方法。
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