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Signed‐For Delivery in the Mitochondrial Matrix: Confirming Uptake into Mitochondria
Small Methods ( IF 10.7 ) Pub Date : 2018-01-23 , DOI: 10.1002/smtd.201700297 Kurt Hoogewijs 1 , Andrew M. James 2 , Michael P. Murphy 2 , Robert N. Lightowlers 3
Small Methods ( IF 10.7 ) Pub Date : 2018-01-23 , DOI: 10.1002/smtd.201700297 Kurt Hoogewijs 1 , Andrew M. James 2 , Michael P. Murphy 2 , Robert N. Lightowlers 3
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Mitochondria are best known for their ability to provide energy in the form of an elevated ATP/ADP ratio to the cell via oxidative phosphorylation, and impairment of mitochondrial ATP production can lead to a variety of diseases. For many of these disorders, caused by mutations in the mtDNA, classical gene therapy or gene‐editing techniques are not possible due to the difficulty of importing nucleic acids into the mitochondrial matrix where the aberrant genes are transcribed. Technical progress in this field has been severely hampered by the inability to correctly determine whether uptake into the mitochondrial matrix of nucleic acids and related oligomers has occurred. Here, the methods that have been used to determine translocation of macromolecules into mitochondria in the past are described, and some recent developments in the field are discussed.
中文翻译：
已签署以便在线粒体基质中递送：确认被线粒体吸收
线粒体以能够通过氧化磷酸化以提高的ATP / ADP比的形式向细胞提供能量的能力而广为人知，线粒体ATP产生的损伤可导致多种疾病。对于许多由mtDNA突变引起的疾病，由于难以将核酸导入转录异常基因的线粒体基质中，因此无法进行经典的基因治疗或基因编辑技术。该领域的技术进步由于无法正确确定是否已发生核酸和相关低聚物摄入到线粒体基质中而受到严重阻碍。在此，介绍过去用于确定大分子向线粒体易位的方法，
更新日期：2018-01-23
中文翻译：
已签署以便在线粒体基质中递送：确认被线粒体吸收
线粒体以能够通过氧化磷酸化以提高的ATP / ADP比的形式向细胞提供能量的能力而广为人知，线粒体ATP产生的损伤可导致多种疾病。对于许多由mtDNA突变引起的疾病，由于难以将核酸导入转录异常基因的线粒体基质中，因此无法进行经典的基因治疗或基因编辑技术。该领域的技术进步由于无法正确确定是否已发生核酸和相关低聚物摄入到线粒体基质中而受到严重阻碍。在此，介绍过去用于确定大分子向线粒体易位的方法，
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